氨基末端激酶信号通路在Apoptin基因诱导Hela细胞凋亡中的作用  

The Effect of c-Jun N-terminal Kinase Signal Pathway on Apoptosis Induced by Apoptin Gene in Hela Cells

作　　者：花象锋[1] 聂晶[1] 赵洪礼[1] 

机构地区：[1]沈阳军区联勤部疾病预防控制中心分子生物学实验室

出　　处：《中国肿瘤临床》2005年第18期1028-1030,共3页Chinese Journal of Clinical Oncology

基　　金：辽宁省自然科学基金资助(编号:20042046)

摘　　要：目的:探讨肿瘤特异性凋亡基因(Apoptingene)在诱导Hela细胞凋亡时,c-Jun氨基末端激酶(JNK)信号通路在细胞发生凋亡中的作用。方法:用含有肿瘤特异性凋亡(Apoptin)基因的真核表达载体瞬间转染体外培养的Hela;采用流式细胞仪、免疫印迹法(Westernblot)、苔盼蓝染色法及RT-PCR等方法研究其在不同时间条件下Hela细胞诱导凋亡的作用。结果:Apopin基因瞬间转染的Hela细胞可出现明显的细胞凋亡;而且凋亡细胞的数量与Apoptin的转染时间有关;凋亡细胞内磷酸化型JNK蛋白随凋亡细胞数量的增加而增多,而非磷酸化型JNK蛋白表达无明显变化。结论:Apoptin基因具有诱导Hela细胞凋亡的作用,JNK信号通路的激活可能在Apoptin诱导肿瘤细胞凋亡过程中发挥作用。Objective： To explain the role of c-Jun N-terminal kinase （JNK） in the apoptosis induced by apoptin gene in human Hela cell. Methods： The Hela cell was transiently transfected by pcDNAA3 plasmids which contained apoptin gene, and apoptosis was measured by flow-cytometry. JNK was detected using western blotting analysis at various time periods. Results： The apoptin gene could induce apoptosis of Hela cell in vitro at 48h after transfection. The level of phosphorylated form of JNK was increased at 24h and reached peak level at 48h after transfected with pcDNAA3. The mRNA of apoptin gene could be detected by RT-PCR at 24h after transfection. Survival cells were associated with time after transfection. Conclusion： Apoptin gene can induce apoptosis in Hela cell, and JNK can play an important role in the apoptosis induced by apoptin gene in vitro.

关 键 词：肿瘤特异性凋亡基因 细胞凋亡 c—Jun氨基末端激酶 

分 类 号：R73-3[医药卫生—肿瘤]