大孔吸附树脂分离纯化罗汉果皂甙的新方法  

Method for isolation and purification of mogrosides with macroporous resin

作　　者：戚向阳[1] 张俐勤[1] 陈维军[1] 宋云飞 潘思轶[1] 

机构地区：[1]华中农业大学食品科技学院,武汉430070 [2]桂林莱茵生物制品有限公司,桂林541300

出　　处：《农业工程学报》2005年第9期163-166,共4页Transactions of the Chinese Society of Agricultural Engineering

摘　　要：该文对大孔树脂分离纯化高含量罗汉果皂甙提取物的新方法进行了研究。结果表明:大孔树脂D101对罗汉果皂甙的分离效果较好,最佳柱分离条件为:上样液pH值9.0,在40℃下依次用pH值9.0的碱水溶液、蒸馏水、30%乙醇及60%乙醇进行洗脱,将60%乙醇洗脱液浓缩,冷冻干燥即得高含量罗汉果皂甙提取物。高效液相分析表明,罗汉果皂甙中几种主要成分的含量均高于分离前的罗汉果皂甙水提物,其中罗汉果皂甙V的含量为69.24%,提高了41.12%。A new method for isolation and purification of mogrosides with macroporous resin was studied. The results showed that the optimum column isolation condition was as follows ： the aqueous solution of crude extract of momordica grosvenori. （MGC） at pH 9. 0 was loaded onto an D101 resin column, and then washed with pH 9.0 aqueous solution, distilled water, 30% ethanol and 60% ethanol in proper order at 40~C. The eluent of 60% ethanol was collected, concentrated and freeze-dried, the mogroside extract （MGI）was obtained and the contents of its main mogrosides were all more than that of MGC, especially the content of its rnogroside V was 69.24% （HPLC）, increasing by 41.12% over MGC.

关 键 词：罗汉果皂甙 分离 大孔树脂 

分 类 号：TS201.2[轻工技术与工程—食品科学]