高强度超声对兔坐骨神经的组织学影响  

Pathological changes of rabbit sciatic nerve caused by exposure to high intensity ultrasound

作　　者：李信明[1] 魏安宁[2] 陆杰[3] 王燕芹[4] 杜永洪[4] 王智彪[4] 

机构地区：[1]四川省遂宁市人民医院麻醉科,遂宁629000 [2]重庆医科大学第二附属医院麻醉科 [3]重庆医科大学生理教研室 [4]重庆医科大学医学超声工程研究所

出　　处：《中华物理医学与康复杂志》2005年第9期517-519,共3页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家"九五"重点科技攻关项目专题(No.969050201);重庆市卫生局青年科研项目(No.99-3010)

摘　　要：目的观察高强度超声(HIU)作用于兔坐骨神经干后,其组织学变化及转归,为超声治疗临近大神经器官、组织提供与神经保护相关的实验依据,并为拓展超声在周围神经领域的应用提供实验基础。方法以不同的HIU剂量(强度×时间)作用兔坐骨神经干,然后观察作用处各时间点组织形态变化。结果作用0s(即对照组为假照)无明显组织学改变;作用15s(45.86W/cm2×15s)病变轻微,2周恢复;作用25s(45.86W/cm2×25s)病变加重,部分神经纤维轴突断裂、变性,3个月恢复;作用50s(45.86W/cm2×50s)大部分神经纤维凝固性坏死,6个月仍未恢复。结论坐骨神经受损伤程度与剂量大小有关,剂量小,损伤轻;剂量大,损伤重。在一定剂量范围内,受损神经可以完全恢复,剂量过大,受损神经难以恢复。Objective To observe the pathologic changes of the rabbit sciatic nerves after the exposure of high intensity ultrasound（HIU） （7.1 MHz ,45.86 W/cm^2） and to establish the experimental base for protection of the nerves near the organs and tissues treated with HIU. Methods The rabbit sciatic nerves were exposed to HIU of different doses as defined by the action time of the uhrsound（0, 15, 25 and 50 seconds, respectively） ; The Pathological changes of the nerves were observed at different times after exposed to HIU （ 1 week,2 weeks, 1 month,3 months and 6 months）. Results In 0 s （45.86 W/cm^2 × 0 s）group, no significant pathological changes were observed. In 15 s（45.86 W/cm^2 × 15 s） group, slight deformation of the myelin sheath were observed and recovery took place at 2 weeks. In 25 s （45.86 W/cm^2 × 25 s） group, the nerves became broken partly and recovered at 3 months. In 50 s（45.86 W/cm^2 × 50 s） group, great part of the nerves got coagulation necrosis and had not recovered at 6 months. Conclusion The larger the dosage of HIU, the higher degree the injury of the sciatic nerve, the more difficult the nerves to get repaired.

关 键 词：高强度超声 坐骨神经 损伤与修复 实验研究 兔 坐骨神经干 组织学变化 剂量范围 组织形态变化 损伤程度 

分 类 号：R312[医药卫生—基础医学]