酶免疫试验检测牛肉中污染的牛中枢神经组织的研究  

Detection of beef contaminated by bovine central nervous system tissue by enzyme immunoassay

作　　者：李冰玲[1] 马贵平[1] 李炎鑫[1] 田海燕[1] 

机构地区：[1]北京出入境检验检疫局疯牛病检测实验室,北京100029

出　　处：《中华实验和临床病毒学杂志》2005年第3期286-289,共4页Chinese Journal of Experimental and Clinical Virology

基　　金："十五"国家科技攻关项目(2001BA804A12)

摘　　要：目的研究应用酶免疫试验(EIA)检测牛肉中污染的牛中枢神经(CNS)组织的影响因素,并将该方法应用到进口牛肉和国产牛产品的检测中。方法对含有不同浓度中枢神经组织的牛肉匀浆分别进行加热处理和不加热处理,放置不同时间后用RIDASCREEN(r)RiskMaterial10/5和RIDASCREEN(r)ProbennahmezubehorSamplingtools试剂盒进行检测;对与标准相同浓度脑组织的PBS悬液和样品稀释缓冲液(SDB)悬液用试剂盒进行检测;对美国进口牛肉及国产市售牛产品中是否含有中枢神经组织进行检测。结果该试剂盒可检出生肉产品和加热处理的肉产品中的中枢神经组织。样品的吸光度值随着中枢神经组织含量的增加而增加;加热和放置时间的延长可使样品的吸光度值下降;脑组织的PBS悬液比相应浓度的样品稀释缓冲液(SDB)悬液的吸光度值高,二者的吸光度值都比相应浓度的标准高。进口牛肉的检测结果均为阴性;国产牛产品除牛尾为阳性外其余样品均为阴性。结论酶免疫法用于检测牛肉中污染的中枢神经组织具有很高的灵敏性;加热和放置时间延长可使样品的吸光度值下降。如果屠宰过程不当,会造成牛产品被中枢神经组织成分污染。Objective To study influencing factors of detection of bovine central nervous system （CNS） tissue contaminated beef by enzyme immunoassav （EIA）, and the method was applied to the detection of imported beef and domestic beef of China. Methods Raw beef homogenates containing different concentrations of raw CNS tissue and the same samples which were heated were detected after different time by RIDASCREEN （r） Risk Material 10/5 and RIDASCREEN（r） Probennahme- zubehor Sampling tools kits. PBS suspension and sample dilution buffer （SDB） suspension of bovine brain tissue with the same concentration of the standard were detected. Beef from USA and domestic market of China were then detected by the kits. Results The kits could detect both raw and heated CNS tissue in the products with high sensitivity. The absorbance values （AV） increased with the concentrations of CNS in samples. Heating and increasing of time could decrease the absorbance values of the samples which contain CNS tissue. The AV of the PBS suspension of bovine brain tissue was higher than the SDB suspension and the AV of both were higher than the AV of standard of the same concentration. No CNS tissue was detected from all imported beef. No CNS tissue was detected in all samples from domestic market of China except for oxtail. Conclusions The EIA method has high sensitivitv for detection of bovine CNS tissue contaminated beef with the glial fibrillary acidic protein （GFAP） as accurate target subslance. Heating and increasing of time can lead to decreasing of the AV of samples. Improper slaughter process can lead to contamination of bovine products by bovine CNS tissue.

关 键 词：脑病 牛海绵状 危害物质 免疫酶技术 中枢神经组织 检测结果 酶免疫试验 牛肉 污染 加热处理 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]