大肠癌患者手术前后血循环中癌细胞CK20m RNA检测的临床意义  

Significanq of detection of circulating cancer cells in the peripheral blood during colorectal cancer resection

作　　者：陈慧玉[1] 周连锁[1] 唐强[1] 金灌培[1] 刘菁[1] 

机构地区：[1]江苏省连云港市第一人民医院肿瘤外科,连云港222002

出　　处：《陕西医学杂志》2005年第10期1190-1192,共3页Shaanxi Medical Journal

基　　金：广东省医学科学技术研究基金(A2001453);江苏省连云港市科委学科带头人基金(QN200202)

摘　　要：目的:研究手术对大肠癌患者血循环中癌细胞的影响。方法:应用RT-PCR技术,检测82例大肠癌患者术前和术后血循环中CK20mRNA的表达。结果:在所有大肠癌患者血循环中术前有42.7%的患者CK20mRNA表达阳性,CK20mRNA阳性检出率与Dukes分期、淋巴结转移、肝转移有显著性差别。手术后在所有大肠癌血循环中有59.8%的患者CK20mRNA表达阳性,较术前阳性表达明显增多(P<0.05)。结论:血循环中癌细胞的存在是大肠癌的一种常见的早期事件,手术刺激可增加肿瘤细胞向血循环中释放。Objective： To study the effect on cancer ceils in the portal vein blood during colorectai cancer resection. Methods： Cytokeratin 20 mRNA in peripheral blood of 82 patients with colorectai carcinoma was detected by reverse transcriptase-polymerase chain reaction （RT-PCR） during colorectai cancer resection. Results： The positive rate of CK20mRNA in peripheral blood was 42.7%. The positive detection was significiantly correlated with Duckes stage, lymph node metastasis and liver metastasis, The positive rate of CK20mRNA in peripheral blood was 59.8% after operation, singnificently higher than before operation （P〈0.05） . Conclusion： Molecular detection in peripheral blood at surgery suggests that hematogenic tumor cell dissemination is a common and early event and that surgical manipulation enhances this release of tumor ceils into the circulation.

关 键 词：大肠肿瘤 角蛋白 血液循环 外科手术 CK20MRNA 大肠癌患者 mRNA检测 手术前后 血循环 癌细胞 

分 类 号：R735.34[医药卫生—肿瘤] R373.9[医药卫生—临床医学]