rab5a基因在肿瘤转移中的作用研究  

The Roles of rab5a Gene in the Mechanism of Tumor Metastasis

作　　者：史忠诚[1] 于旸[1] 李钰[1] 傅松滨[1] 

机构地区：[1]哈尔滨医科大学医学遗传学研究室,哈尔滨150086

出　　处：《遗传》2005年第5期694-698,共5页Hereditas（Beijing)

基　　金：国家自然科学基金(编号:30170516;39970396);国家自然科学基金国际合作项目(编号:30213023);黑龙江省卫生厅科研课题项目基金(编号:2003-105)~~

摘　　要：为研究rab5a基因在肿瘤转移机制中的作用,将该基因稳定转染至低转移肺腺癌细胞系AGZY83-a中,采用Superarray肿瘤转移相关基因微芯片分析rab5a对肿瘤转移相关基因的表达影响。共获得了5个差异表达基因,rab5a基因促进s100a4的表达,同时抑制了nm23ar、ac1、cst3、col4a2等基因的表达,并分别在RNA及蛋白水平进行验证,确认rab5a基因影响了肿瘤转移的多个途径,促进了肿瘤细胞转移能力增强。To study the role of rab5a gene in the into AGZY83-a cell, a lung adenocarcinoma cell li mechanism of tumor metastasis, ne with low metastatic potential rab5a gene was stable transfected Superarray was applied to study the effect of rab5a on the differential expression of genes related to tumor metastasis. Five differentially expressed genes were obtained, including one up-regulated gene s100a4 and 4 down-regulated genes, nm23a, racl, cst3 and col4a2. These results were confirmed at both the RNA and protein levels. We concluded that rab5a promoted tumor metastasis by affecting multiple genes in several pathways involved in tumor metastasis.

关 键 词：RAB5A基因 肿瘤转移 微芯片 

分 类 号：R730.2[医药卫生—肿瘤]