Staufen基因在结直肠癌组织中的表达  

The Expression of Staufen Gene in Colorectal Cancer

作　　者：朱益民[1] 林洁[2] 陈俭[2] 黄琼[2] 邵丽娜[2] 来茂德[2] 

机构地区：[1]浙江大学医学院公共卫生系,杭州310006 [2]浙江大学医学院病理学教研室,杭州310006

出　　处：《遗传》2005年第5期705-709,共5页Hereditas（Beijing)

基　　金：国家自然科学基金(编号:30070343)~~

摘　　要：为研究甲基化差异相关基因Staufen在结直肠癌不同组织中的表达情况,采用RT-PCR和免疫组织化学等方法在结直肠癌病人中检测Staufen基因在腺癌、癌旁黏膜和相应远端切缘正常组织中的表达。研究发现,在mRNA水平上,远端切缘正常组织中Staufen基因的表达水平显著高于癌旁黏膜和腺癌(P<0.05);从正常组织、癌旁黏膜到腺癌,Staufen基因的表达有降低的趋势;未发现性别、年龄、肿瘤部位(结肠/直肠)、分化程度、淋巴结转移等临床病理因素与Staufen基因的表达有关(P>0.05)。正常组织与腺癌组织的Staufen蛋白的表达水平显著高于癌旁黏膜(P<0.05),而正常组织与腺癌组织之间未发现有统计学差别(P>0.05)。结果表明,Staufen基因在癌旁黏膜和肿瘤中有低表达的趋势,该基因可能在结直肠癌的发生、发展中起作用。To study the expression of differential methylation - associated Staufen gene in colorectal cancer（ORC）, we detected the expression of Staufen gene in adenocarcinoma, mucosa adjacent to colorectal cancer（MAOO）and corresponding distant normal tissue using RT-PCR and immunohistochemistry. We found that the expression of Staufen gene in MACC and adenocarcinoma of ORC was significantly lower than that in corresponding distal normal tissue at mRNA level（P〈0.05）. No significant associations were found between age, sex, location of cancer （colon/ rectal）, degree of differentiation and metastasis of lymph node with expression of Staufen gene. Lower expression of Staufen protein in MACC was verified by comparing with matched normal tissue and adenocarcinoma（P〈0.05）. This study indicated low expression of Staufen gene in colorectal cancer and the Staufen gene might be involved in the development of colorectal cancer.

关 键 词：结直肠癌 Staufen基因 RT—PCR 免疫组化 

分 类 号：R735.37[医药卫生—肿瘤]