一种三维血管瘤血管生成体外培养模型的建立  

A three-dimension in vitro model for angiogenesis of hemangioma

作　　者：江成鸿[1] 庄福连[2] 黄拔瑞[1] 郭志辉[1] 谢义德[1] 周亚宽[1] 王彪[2] 陈文列[3] 

机构地区：[1]福建医科大学附属协和医院整形外科,福州350001 [2]福建医科大学附属第一医院整形外科 [3]福建医科大学电镜室

出　　处：《中华整形外科杂志》2005年第5期364-367,共4页Chinese Journal of Plastic Surgery

基　　金：福建省青年科研基金资助项目(2004110)

摘　　要：目的建立一种三维血管瘤血管生成体外培养模型。方法将新鲜的增殖期血管瘤手术标本置于纤维蛋白凝胶,以双层夹心法建立三维血管瘤血管生成体外培养模型。结果血管瘤组织培养2～3d后芽生出细小血管,后呈枝丫状生长,至第8～9天长成树枝状血管,分叉交叠,之后血管生长渐缓停滞。组织周边新生树枝状结构经石蜡切片HE染色、血管内皮细胞特异性标志物第Ⅷ因子相关抗原检测及电镜观察,鉴定为血管。结论该模型的成功建立有助于血管瘤血管生成机制及抗血管生成药物的研究。Objective To create a three dimension（3D） in vitro model fnr angiogenesis of hemangioma. Method The fragment of hemangioma speeimen was embedded in fibrin gel to set up the three-dimesnsion（3D） in vitro rondel for angingenesis of hemangioma. Result In the model, microvessels grew out from the tissue fragments at the 2nd to 3rd day after culture, and at the 8th to 9th day a compact network of microvessels come into being,then tending to be stationary . The eompact network around the tissue fragment was confirmed to be blood vessels by immunohistochemistry and electrott microscopy. Conclusion This model helps to study the mechanism of hemangioma angingettesis and investigate the drugs of anti- angiogenesis.

关 键 词：血管瘤 血管生成 纤维蛋白凝胶 体外培养模型 抗血管生成药物 体外培养模型 血管瘤 三维 纤维蛋白凝胶 特异性标志物 血管内皮细胞 组织培养 血管生长 

分 类 号：R-331[医药卫生]