乙酰肝素酶反义寡核苷酸对人胃癌细胞株SGC7901表达bFGF的抑制作用  

Inhibition of expression of basic fibroblast growth factor in human gastric carcinoma cell line SGC 7901 by heparanase antisense oligodeoxynucleotide

作　　者：田斌[1] 周世庆[2] 汪素文[2] 真岩波[2] 张淑红[2] 

机构地区：[1]山东大学临床医学院济南市中心医院普通外科,山东济南250013 [2]山东大学临床医学院济南市中心医院消化内科,山东济南250013

出　　处：《肿瘤防治杂志》2005年第19期1469-1471,共3页China Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨乙酰肝素酶反义寡核苷酸对人胃癌细胞株SGC7901细胞中表达碱性成纤维细胞生长因子(basic fibro-blast growth factor,bFGF)的影响。方法:用脂质体介导乙酰肝素酶反义寡核苷酸转染人胃癌细胞株SGC7901细胞,采用免疫细胞化学观察细胞内bFGF的变化。结果:脂质体介导转染乙酰肝素酶反义寡核苷酸前后,SGC7901胃癌细胞内乙酰肝素酶mRNA积分光密度值(integ-ra optical densit,IOD)分别为256·32±3·21、146·89±2·35、85·36±3·58和63·78±1·42。bFGF阳性表达率转染前72·23%,转染0·2、0·4和0·8μmol/L的AS-ODN后分别为57·15%、51·11%和42·36%。与转染前比较,转染后细胞bF-GF表达明显减少,P值分别为0·007、0·003和0·001。结论:转染乙酰肝素酶反义寡核苷酸可以有效抑制胃癌细胞bF-GF的表达。肿瘤防治杂志,2005,12(19)OBJECTIVE： To evaluate the effect of heparanase antisense oligodeoxynucleotide （AS-OND） on the expression of basic fibroblast growth factor （bFGF） in human gastric carcinoma cell line SGC 7901. METHEDS：AS-ODN of heparanase mRNA and nonsense oligodeoxynucleotide （NS-ODN） were designed and synthesized and phosphorothioated. The ODNs were embedded in cationic liposome lipofectinand transfected into SGC 7901 cells. The total RNAs were extracted from the cells 48 hours after transfection and the ,semiquantitative RT-PCR were performed to evaluate the heparanase gene. The change of bFGF was observed by using immunocytochemistry. RESULTS：The beparanase gene expression and the expression of bFGF of SGC7901 cells treated with AS-ODN of different final concen trations were significantly decreased compared with that of the controls （P were 0. 007,0. 003,0. 001 respectively）. The expression rates were 72.23%. 57. 15%, 51.11% and 42.36% at cells before and after treated with AS-ODN of the final concentrations of 0.2, 0.4 and 0. 8 μmol/ L, respectively. CONCLUSION： Heparanase AS-ODN complementary to the start codon region of heparanase mRNA has a significant effect on the expression of bFGF in human gastric carcinoma cell line SGC 7901 in a dose-depended manner.

关 键 词：胃肿瘤 寡核苷酸类 反义 成纤维细胞生长因子 碱性 肝素 RNA 信使/遗传学 逆转录聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤]