HPLC-MS^2鉴定大青叶水提液中的5种化学成分  

Identification of 5 Constituents of the Aqueous Extract of Isatis indigotica by HPLC-MS^2

作　　者：刘瑞[1] 袁波[1] 刘志刚[1] 李小芩[1] 熊志立[1] 李发美[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中药材》2005年第9期772-774,共3页Journal of Chinese Medicinal Materials

基　　金：国家科技攻关计划课题(2001BA701A56)子课题

摘　　要：目的:鉴定大青叶水提液中的化学成分。方法:大青叶的水提液经HPLC分离后,收集各色谱峰的馏分进行二级质谱分析,并和胞苷、尿苷、鸟苷、黄嘌呤和次黄嘌呤对照品的水溶液进行比较。结果:大青叶水提液HPLC中的5个色谱峰分别与胞苷、尿苷、鸟苷、黄嘌呤和次黄嘌呤对照品的t_R和UV光谱一致,并具有相同的二级质谱碎片离子。结论:大青叶中含有胞苷、次黄嘌呤、尿苷、黄嘌呤和鸟苷等5种成分。Objective ：To identify five constituents in the aqueous extract of l.satis indigotica Fort. Methods ： After separation of the aqueous extract of lsatis indigotica Fort. by HPLC,the eluates of five peaks were collected separatively and analysed by MS^2. UV spectra and MS2 were compared with those of reference standards of cytidine, uridine, guanosine xanthine and hypoxanthine. Results：Each HPLC elute of the aqueous extract had same retention time, UV spectra and fragment pattern in the MS^2 spectrum as the corresponding standards. Conclusion：Five constituents of the aqueous extract of lsatis indigotica Fort. are identified as cytidine, hypoxanthine, uridine, xanthine and guanosine.

关 键 词：大青叶 胞苷 尿苷 鸟苷 黄嘌呤 次黄嘌呤 HPLC—MS^2 HPLC分离 化学成分 水提液 

分 类 号：R977.15[医药卫生—药品] R284.1[医药卫生—药学]