益气明目口服液对光损伤大鼠视网膜电图的影响  

Effects of Yiqimingmu Solutions on Electroretinogram in Rats with Retinal Photochemical Damage

作　　者：王延东[1] 唐细兰[1] 吴伟[1] 叶成添[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《中药材》2005年第9期805-808,共4页Journal of Chinese Medicinal Materials

基　　金：广东省中医药局科研基金(102070)

摘　　要：目的:观察中药益气明目口服液对大鼠视网膜光化学损伤后视网膜电图的影响。方法:24只SD大鼠随机分为阴性对照组、模型组、低剂量组、高剂量组。除阴性对照组外,各组实验大鼠均接受1900±106.9 Lux绿色荧光灯24h持续光照射,建立大鼠视网膜光化学损伤模型。低剂量组与高剂量组于光照前7d分别予以不同剂量益气明目口服液灌胃给药,阴性对照组及模型组给予等量生理盐水灌胃。观察光照后6h、6d、14d各组视网膜电图中a波及b波的变化。结果:阴性对照组大鼠左、右眼ERGa波及b波的振幅差异无显著性的意义;大鼠ERGa波及b波的振幅于视网膜光化学损伤后6h及6d均持续降低,至14d时仍无显著恢复;益气明目口服液低、高剂量组对实验大鼠光化学损伤后ERGa波振幅的降低均有保护作用,高剂量组益气明目口服液可有效保护实验大鼠光化学损伤后ERGb波振幅的降低。结论:益气明目口服液对视网膜光化学损伤有良好的保护作用。Objective：To observe the effect of the Yiqimingmu solutions on electroretinogram of rats with retinal photochemical damage. Methods：Twenty-four SD rats were divided into four groups randomly ：control group, model group, low dose group, high dose group. All groups except the control one were continually exposed to green fluoresent light with an level of 1900 ± 106. 9 Lux for 24 hours, which was used to establish rats retinal photochemical damage model. Different doses of Yiqimingmu solutions were respectively irrigated into rats 7 days before light expose in low dose and high dose group. Saline solutions instead was used in control and model group. At 6 hours and 6,14 days after light exposed,change of electroretinogram a wave and b wave was observed. Resuhs：It was suggested that the difference of the average amplitude of ERG a wave and b wave was not significant between left eyes and right eyes in the control group. The average amplitude of ERG a wave and b wave decreased continually at the 6^th hour and 6^th day and uncovered at the 14^th day after light exposed in the model group. In the low dose and high dose group, Yiqimingmu solutions had protective effect on lowing ERG a wave after rats retinal photochemical damage. Conclusion：Yiqimingmu solutions has protective effect on rats retinal photochemical damage.

关 键 词：益气明目口眼液 视网膜 光化学损伤 视网膜电图 SD大鼠 口服液 明目 益气 视网膜光化学损伤 光损伤 

分 类 号：R770.43[医药卫生—眼科] R285.5[医药卫生—临床医学]