HPLC法测定富马酸比索洛尔氢氯噻嗪片中富马酸比索洛尔和氢氯噻嗪的含量  

HPLC Determination of bisoprolol fumarate and hydrochlorothiazide of Bisoprolol fumarate in Hydrochlorothiazide Fumarate Tablets

作　　者：牛玉娟 耿欣 张新妹[2] 

机构地区：[1]大连市药品检验所,大连116021 [2]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》2005年第9期1132-1133,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用高效液相色谱法测定富马酸比索洛尔氢氯噻嗪片中富马酸比索洛尔和氢氯噻嗪的含量。方法:以 C_(18)为色谱柱(5μm,4.6 mm×150mm),以磷酸盐缓冲溶液一乙腈(75:25)为流动相,柱温为40℃,检测波长为220 nm。结果:富马酸比索洛尔线性范围为25-300μg·mL^(-1),(r=0.9998),氢氯噻嗪线性范围为50-200μg·mL^(-1),(r=0.9965)。结论:方法简便,结果准确,适用于该产品的质量控制。Objective：To establish a HPLC method for determination of Bisoprolol fumarate and Hydrochlorothiazide Tablets. Method：Using a C18 column（5μm,4.6mm×150mm） , the mobile phase consisted of phosphate buffer - acetonitrile （75： 25 ）, the detection was carried out at 220 nm. Results： The calibration curves for Bisoprolol fumarate were linear between 25 and 300μg·mL^-1, （ r = 0. 9998 ） , The calibration curves for Hydrochlorothiazide were linear between 50 - 200μg·mL^-1, （ r = 0. 9965 ） o Conclusion ： This method is convenient and accurate.

关 键 词：HPLC 富马酸比索洛尔 氢氯噻嗪 含量 氢氯噻嗪片 HPLC法 测定 高效液相色谱法 磷酸盐缓冲溶液 线性范围 

分 类 号：R972[医药卫生—药品] R972.4[医药卫生—药学]