转染野生型p53基因对肝癌细胞生长的影响  

EFFECT OF p53 GENE TRANSFECTION ON HEPATOCARCINOMA CELL

作　　者：赖祥进[1] 曹林枝[1] 廖志红[1] 刘波[2] 翁国庆[3] 

机构地区：[1]广西医科大学生物化学与分子生物学教研室,南宁530021 [2]广西医科大学第一附属医院临床医学实验中心 [3]钦州市第一人民医院

出　　处：《广西医科大学学报》2005年第4期489-491,共3页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助项目(No.桂科自9912035)

摘　　要：目的:通过转染野生型p53基因致肝癌细胞株BEL-7404,研究其对肝癌细胞的生长及凋亡作用。方法:野生型p53基因通过脂质体Lipofectamine介导转染人肝癌细胞株BEL-7404。用四甲基偶氮唑(MTT)法测定细胞生长曲线,用流式细胞仪进行细胞凋亡分析。结果:肝癌细胞BEL-7404转染野生型p53基因后,其生长作用明显受到抑制,细胞生长抑制率在第4天可达50.8%。流式细胞仪分析细胞凋亡结果:阴性对照组为1.7%,转染pUHD10-3组为3.2%,转染pUHD10-3-p53组为40.7%。结论:野生型p53基因可诱导肝癌细胞BEL-7404的生长抑制及发生凋亡。Objective. To investigate the effect of p53 gene transfection on the growth and apoptosis ofhepatocarcinoma cell line BEL-7404. Methods： The HCC line BEL-7404 was transfected with plasmid pUHD10-3 recombined with wt-p53 gene mediated by Lipofectamine. 3-（4, 5-Dimethy-2-thiazolyl ）-2, 5-diphenyl-2H-tetrazolium-bromid （MTT） technique was used to analyse the growth of HCC cells. Theapoptosis of the cells was evaluated by flow cytometry. Result：The growth of HCC cell line BEL-7404 wasinhibited after transfected with wt-p53 gene, and 50.8% of the cells were inhibited on the fourth day. The rates of apoptosis measured by flow cytometry were： 1. 7% in the untreated group, 3. 2%o in the group transfected with pUHD10-3, 40. 7% in the group tranfected with pUHD10-3-p53. Conclusion： The inhibition efficiency and apoptosis of hepatocarcinoma cell line BEL-7404 was induced by wt-p53 gene.

关 键 词：肝癌 P53基因 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]