胃癌前病变及胃癌组织中survivin和caspase-3蛋白表达的意义  

Significance of survivin and caspase-3 protein expression in gastric precancerous lesions and gastric cancer tissue

作　　者：吕晓君[1] 吴东瑛[1] 杨琳[2] 张淑敏[1] 辛彦[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所第四研究室,辽宁省沈阳市110001 [2]中国医学科学院中国协和医科大学肿瘤医院病理科,北京市100021

出　　处：《世界华人消化杂志》2005年第16期1951-1955,共5页World Chinese Journal of Digestology

基　　金：国家自然基金项目资助项目;No.30371607~~

摘　　要：目的:检测survivin和caspase-3蛋白在胃癌前病变及胃癌组织中的表达情况,探讨其与胃黏膜癌变的关系及作用机制.方法:采用Envision免疫组化学二步法检测不同胃黏膜病变组织131例(包括慢性炎症44例、单纯肠化生31例,异型增生40例和胃癌16例)中survivin和caspase-3蛋白的表达情况.结果:慢性炎症,单纯肠化生,异型增生和胃癌中的survivin蛋白阳性表达率分别为4.5%(2/44),51.6%(15/31),100.0%(40/40)和93.8%(15/16).胃癌组织和异型增生组survivin蛋白表达显著高于单纯肠化生组(P<0.05).胃癌survivin蛋白表达阳性组caspase-3蛋白表达(3/13,23.1%)显著低于survivin蛋白表达阴性组(2/3,66.7%,P<0.05).结论:胃癌survivin蛋白表达水平与异型增生相当,高于单纯肠化生,survivin可能通过阻抑caspase-3促进胃癌的演进.AIM： To detect the expression survivin and caspase-3 in the tissues of gastric cancer and precancerous lesion, and to explore their roles in the carcinogenesis gastric cancer. METHODS： The expression of survivin and caspase-3 protein were detected by Envision immunohistochemistry in 131 cases of paraffin-embedded specimens from different gastric mucosa, including 44 cases of chronic gastritis, 31 cases of intestinal metaplasia, 40 cases of dysplasia and 16 cases of gastric cancer. RESULTS： The posftive rates of survivin express（on in chronic gastritis, intestinal metaplasia, dysplasia and gastric cancer were 4.5%（2/44）, 51.6%（15/31 ）, 100.0%（40/40） and 93.8%（15/16） respectively. The positive rates of survivin protein in gastric cancer and dysplasia were significantly higher than that in intestinal metaplasia（P〈0.05）. The levelof caspase-3 expression in positive survivin groups was significantly lower than in negative survivin group（23.1% vs 66.7%, P〈0.05）. CONCLUSION： The positive rate of survivin protein in gastric cancer is similar to that in dysplasia, and higher than that in intestinal metaplasia. Survivin may promote the progression of gastric cancer by inhibiting the expression of caspase-3.

关 键 词：胃癌 SURVIVIN Caspase-3 癌前病变 CASPASE-3蛋白 SURVIVIN蛋白 蛋白表达水平 胃癌组织 胃癌前病变 ENVISION 

分 类 号：R735.2[医药卫生—肿瘤]