猕猴肝纤维化模型建立与评价  

Establishment and evaluation of macaque liver fibrosis model

作　　者：庞荣清[1] 刘建昆[1] 何占龙[2] 王惠萱[1] 张步振[1] 王鹤桦 杨勇琴[1] 屈璐[1] 潘兴华[1] 

机构地区：[1]成都军区昆明总医院医学实验科,云南省昆明市650032 [2]中国医学科学院协和医科大学昆明医学生物研究所,云南省昆明市650032

出　　处：《世界华人消化杂志》2005年第16期1956-1958,共3页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助;No.30270674云南省自然科学基金重点资助项目;No.2003C013Z~~

摘　　要：目的:探讨灵长类动物肝纤维化模型制作方法,建立与人类肝纤维化更接近的动物肝纤维化模型.方法:猕猴10只,随机分为实验组和对照组,实验组每周皮下注射400mL/LCCl40.8mL/kg,连续10wk,同时辅以高脂饲料饲喂.注射CCl4后11wk,检测肝功相关的血清蛋白、血脂浓度及观察肝组织病理学变化.结果:实验组血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、球蛋白(GLO)、血清总胆固醇酯(TCH)、三酰甘油(TG)、低密度脂蛋白(LDL)、血清胆红素(TBIL)、血清透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原含量与对照组相比均显著升高(2017.1±244.5nkat/Lvs550.1±72.8nkat/L,1978.6±237.0nkat/Lvs488.9±55.2nkat/L,47.7±5.4g/Lvs41.5±6.1g/L.4.2±0.5mmol/Lvs3.3±0.4mmol/L,1.1±0.2nmml/Lvs0.5±0.1mmol/L,1.9±0.3mmol/Lvs1.4±0.2mmol/L,9.5±1.2μmol/Lvs6.3±0.8μmol/L,419.3±42.1μg/Lvs68.4±8.2μg/L,274.6±32.2μg/Lvs39.4±4.5μg/L,269.7±18.2μg/Lvs103.7±12.3μg/L,P<0.01),而总蛋白(TP)和白蛋白含量降低与对照组相比显著降低(74.3±8.4g/Lvs84.3±7.7g/L,34±4.3g/Lvs41.7±4.8g/L,P<0.01).纤维化动物肝组织显示,肝小叶间纤维增生,部分纤维细胞向肝细胞间延伸.AIM： To establish the macaque model of liver fibrosis. METHODS： Ten macaques of 7-10 years old were randomly divided into the experiment and control group. The model of liver fibrosis was induced in the macaques of experiment group by intravenous injection of 400 mL/L CCI4 （0.8 mL/kg） weekly for 10 weeks. Meanwhile, the macaques in experiment group were fed with high fat food. The liver function related proteins, enzymes and lipids in serum were detected by automatic biochemical analyzing equipment and the histological changes were investigated by pathological method.lesterol （TCH）, triglycerides （TG）, low density lipoprotein （LDL）, total bilirubin （TBIL）, hyaluric acid （HA）, type Ⅲ procollagen （PCⅢ） and type Ⅳ collagen （IVC） contents in the macaques of experiment group were significantly increased as compared with those of the control group （2 017.1±244.5 nkat/L vs 550.1 ±72.8 nkat/L, 1 978.6± 237.0 nkat/L vs 488.9 ± 55.2 nkat/L, 47.7 ± 5.4 g/L vs 41.5 ± 6.1 g/L, 4.2 ± 0.5 mmol/L vs 3.3 ± 0.4 mmol/L, 1.1 ± 0.2 mmol/L vs0.5 ± 0.1 mmol/L, 1.9 ± 0.3 mmol/L vs 1.4 ± 0.2 mmol/L, 9.5 ± 1.2 μmol/L vs 6.3 ± 0.8 μmol/L, 419.3±42.1μg/L vs 68.4± 8.2μg/L, 274.6±32.2 μg/L vs 39.4 ± 4.5 μg/L, 269.7 ± 18.2 μg/L vs 103.7 ± 12.3 μg/L, all P〈0.01 ）, while the serum total protein （TP） and albumin （ALB） contents was significantly reduced （74.3 ± 8.4 vs 84.3 ± 7.7, 34.0 ± 4.3 vs 41.7 ± 4.8, both P〈0.01. Pathological examination showed that the degeneration and necrosis of hepatocytes appeared, and the fibrotic tissues coagulated and proliferated. CONCLUSION： The macaque model of liver fibrosis can be established successfully by intravenously injecting 400 mL/L CCl4 while giving high fat food for 10 weeks.

关 键 词：猕猴 肝纤维化 模型 肝纤维化模型 谷丙转氨酶(ALT) Ⅲ型前胶原(PCⅢ) mol/L 血清谷草转氨酶 灵长类动物 高脂饲料饲喂 

分 类 号：R-332[医药卫生]