百草枯对小鼠黑质多巴胺能神经元的选择性损害  

Selective Damage of Paraquat on Dopaminergic Neurons in Substanti Nigra of Mice

作　　者：任今鹏 任惠民[1] 蒋雨平 

机构地区：[1]复旦大学附属华山医院神经内科 [2]上海交通大学附属第六人民医院神经内科200232

出　　处：《中国临床神经科学》2005年第3期230-235,238,共7页Chinese Journal of Clinical Neurosciences

基　　金：上海市科学技术发展基金资助课题(004119005)

摘　　要：目的:探讨百草枯对黑质部多巴胺能神经元的损害是否具有选择性。方法:用口服百草枯的途径,建立小鼠帕金森病模型;应用原位杂交技术观察DAT和VMAT2在不同脑区的mRNA表达水平并测定两者的比值,采用细胞计数观察百草枯干预后多巴胺能神经元的损害。结果:在黑质部DATmRNA和VMAT2mRNA的比值最高;在红核和下丘脑室旁核区域DATmRNA与VMAT2mRNA的比值和黑质部相比较低(P<0·01)。百草枯干预后黑质部的多巴胺能神经元减少37·7%;红核和下丘脑室旁核区的多巴胺能神经元分别减少12·2%和13·1%。结论:百草枯对黑质部多巴胺能神经元的损害具有相对选择性,其原因可能与黑质部DATmRNA和VMAT2mRNA的比值高有关。Aim： To explore whether paraquat could damage dopaminergic neurons selectively in substantia nigra of parkinsonian mice induced by paraquat taken orally.Methods： In situ hybridization technique was applied to observe the mRNA expressions of DAT and VMAT 2 in different areas of brain. The ratio of DAT mRNA to VMAT 2 mRNA and the degree of neurons injury in different areas of brain were also observed after the paraquat treatment. Results： It was found that the ratio of DAT mRNA to VMAT 2 mRNA was the highest in substantia nigra, whereas lower in red nucleus and paraventricular hypothalamic nucleus. The dopaminergic neuron was decreased by 37.7 % in substantia nigra whereas 12.2% and 13.1% respectively in red nucleus and paraventricular hypothalamic nucleus after paraquat given orally for two months. Conclusion： The results indicated that the loss of dopaminergic neurons in substantia nigra induced by paraquat may be selective, which was possible related to a higher ratio of DAT mRNA to VMAT 2 mRNA in substantia nigra.

关 键 词：帕金森病 百草枯 多巴胺转运体 囊泡单胺转运体2 

分 类 号：R742.5[医药卫生—神经病学与精神病学]