大脑中动脉梗死模型制作方法的研究  

Evaluation of an Improved Model of Middle Cerebral Artery Occlusion in the Rat by Intraluminal Suture

作　　者：温仲民[1] 包仕尧[1] 

机构地区：[1]苏州大学附属第二医院神经内科,215004

出　　处：《中国临床神经科学》2005年第3期304-306,共3页Chinese Journal of Clinical Neurosciences

摘　　要：目的:通过大鼠短暂性大脑中动脉阻塞后梗死体积的测量和神经功能评分,评价改良大鼠大脑中动脉阻塞模型的可靠性。方法:分别使用包被有多聚-L-赖氨酸(0·1%)尼龙线和普通尼龙线,线栓法制作大鼠大脑中动脉阻塞模型,缺血2h后再灌注,分别在再灌注后3、24、48和72h联合应用八分法和姿势反射评价运动神经功能,3d后处死大鼠,测定梗死体积并进行比较。结果:运动神经功能评分结果与梗死体积成线性关系,多聚-L-赖氨酸包被线组制作模型的成功率高于普通线组,且梗死体积比普通线组大。结论:神经功能评分能很好的反映脑梗死的严重程度;采用多聚-L-赖氨酸包被的尼龙线能使大鼠大脑中动脉阻塞模型更可靠,成功率高,梗死体积和部位变异性小。Aim：To evaluate a modified method of intraluminal suture occlusion of the middle cerebral artery （MCA） on the neurobehavioral function and on the volume of brain infarction in rats subjected to a temporary focal cerebral ischemic insult. Methods： Sprague-Damley rats were subjected to 2 h temporary MCA occlusion （MACO） with poly-L-lysine coated suture （0.1%）and conventional suture. Behavioral motor functions were evaluated at 180 min, 24, 48, and 72 h after reperfusion by eight-point behavioral rating scale and postural reflex. Brains were perfusion-fixed and infarct volume were measured at the time of 72 h after reperfusion. The results were compared between two groups. Results：9 of 20 tats with uncoated suture had infarct, while in the group with poly-L-lysine-coated suture, 15 of 20 rates had infarct. Our results demonstrate a liner relationship between motor functional impairment and lesion volume. Infarct volumes were significantly larger in rats with poly-L-lysine-coated suture group than without poly-L-lysine-coated suture group.Infarct volume in the uncoated sutures tended to be smaller and more inconsistent compared with the group in which sutures were coated with poly- L-lysine. Conclusion： Eight-point behavioral rating scale and postural reflex tests of motor performance provide quantitative, objective measures of functional impairment of rats following an ischemic insult. MCAO in which a poly-L-lysine-coated intralnmianl suture used was proved to ire a reliable and effective modification of this technique.

关 键 词：动物模型 大脑中动脉阻塞 大鼠 多聚-L-赖氨酸 

分 类 号：R743[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]