c-fos癌基因在雌激素诱导的大鼠催乳素瘤中的表达  

Expression of c-fos oncogene in estrogene induced rat prolactinomas

作　　者：陈伟良 张韶峰[2] 徐春[2] 

机构地区：[1]武警浙江总队嘉兴医院内二科,浙江嘉兴314000 [2]武警总医院内分泌科,北京100039

出　　处：《肿瘤防治杂志》2005年第15期1135-1138,共4页China Journal of Cancer Prevention and Treatment

基　　金：首都医学发展科研基金(中医药)(2000-Ⅲ-21)

摘　　要：目的研究c-fos癌基因在雌激素诱发的大鼠催乳素(prolactin,PRL)瘤中的表达,以及多巴胺受体激动剂诺果宁对其表达的影响。方法1)制备雌激素诱发的大鼠PRL瘤模型;2)放免法测定大鼠血清PRL水平;3)垂体称重并做组织病理学观察,用免疫组化方法显示垂体组织PRL蛋白的表达和分布;4)用RT-PCR方法检测c-fos mRNA在各组垂体组织中的表达,以β-actin作为内参照,借助于计算机凝胶成像系统分析表达量。结果用药8周后,PRL瘤组大鼠血清PRL水平、垂体质量以及垂体PRL(+)细胞计数均明显高于对照组,t值分别为32.63、29.77和25.27,P值分别为3.7×10-14、1.2×10-13和9.7×10-13,根据大鼠垂体质量以及组织学和免疫组化的改变,证实已诱发出PRL瘤模型。在PRL瘤组中,c-fos的表达量明显高于对照组,t=39.70,P=2.96×10-15;诺果宁组c-fos表达量较PRL瘤组有所降低,两者差异有统计学意义,t=-16.01,P=9.2×10-10。结论雌激素刺激垂体PRL细胞表达c-fos癌基因,在雌激素诱导PRL瘤中起一定的作用。雌激素诱导垂体PRL细胞表达c-fos受到下丘脑神经递质和垂体内环境等诸多因素的影响。OBJECTIVE： To study the expression of c-los oncogene in estrogen induced rat prolactinoma and the effect of dopamine agonist, norprolac, on c-los oncogene expression. METHODS： 1） Preparation of rat prolactinoma model： Adult Wistar rats, ovariectomized, were divided into 3 groups at random. Each rat in the control group （n= 8） was subscutaneously implanted with a blank implant. Rats in the prolactinoma group（n= 7） were implanted with estrogencontaining implants. Rats in the norprolac group （n= 7）were implanted with estrogen-containing implants, meanwhile norprolac was orally adminstrated. After 8 weeks, all the animals were executed. 2）Serum prolactin（PRL） levels were measured by RIA method. 3）Each pituitary gland was weighed and collected for histopathological and immunocytochemical examination. 4） C-los mRNA level in pituitary tissue was measured by RT-PCR method in each group. RESULTS： PRL levels pituitary weights,and PRL（＋ ）cell countings in the prolactinoma group were obvious higher than those in the control group, t=32.63, 29.77 and 25.27,P=3.7×10^ -14,1.2×10^-13 and 9.7× 10^-13 , respectively. The prolaetinoma model was induced successfully according to the changes of pituitary weight and immunocytochemistry. The expression levels of c-los mRNA in the prolactinoma group was obviously higher than those in the control group,t=39.70, P= 2.96 × 10^15. C-los mRNA levels in the norprolac group was lower than those in the prolactinoma group, t = - 16.01, P = 9.2 × 10^-10. CONCLUSIONS： Estrogen stimulates c-los oncogene expression, which plays an important role in estrogen induced rat prolactinoma. C-los oncogene expression induced by estrogen is affected by hypothalamus neuro-transmitter.

关 键 词：癌基因 C-FOS 大鼠 催乳素瘤 雌激素 多巴胺激动剂 

分 类 号：R736.4[医药卫生—肿瘤]