β－转化生长因子的提纯及其对内毒素诱发的葡萄膜炎的抑制作用  

PURIFICATIONOFTRANSFORMINGGROWTHFACTOR┐BETAANDITSAP┐PLICATIONFORTHEPREVENTIONANDTREATMENTOFENDOTOXININ┐DUCEDUVEITIS

作　　者：杨培增[1,2] 梁舜薇[1,2] 龚向明[1,2] 谢楚芳[1,2] 黄祥坤[1,2] 赵敏[1,2] 曹心女 原潘 苏华 

机构地区：[1]中山医科大学中山眼科中心 [2]重庆医科大学眼科

出　　处：《中华眼底病杂志》1996年第2期101-104,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金

摘　　要：目的：探讨β－转化生长因子（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒ－β，ＴＧＦ－β）对内毒素诱发的葡萄膜炎（ｅｎｄｏｔｏｘｉｎ－ｉｎｄｕｃｅｄｕｖｅｉｔｉｓ，ＥＩＵ）的预防作用。方法：用生物凝胶Ｐ－６０层析的方法从人血小板提取纯化ＴＧＦ－β，用其处理内毒素对大鼠点眼或腹腔注射，进行临床观察和比较。结果：用生物凝胶Ｐ－６０层析获得了高纯度的ＴＧＦ－β，用其点眼或腹腔注射，使ＥＩＵ的发生推迟约４小时，炎症的严重程度显著减轻、持续时间缩短３天。PURPOSE:Toinvestigatetheinfluenceoftransforminggrowthfactor-beta(TGF-β)onendotoxin-induceduveitis(EIU).METHODS:TGF-βwasabstractedfromhumamplateletsusingBio-gelP-60chromatography.Itwasintroducedeithertopicaly(drops)orintraperi-tonealyintotheSDratsafterfootpadinjectionoflipopolysaccharide(LPS).Theinflammationintheanterioruveawasclinicalyevaluatedwithslitlampeveryday.RESULTS:TGF-βobtainedfromBio-gelP-60chromatographydisplayedsinglebandonSDS-PAGE,showingamolecularweightof12500EIUoccurssignificantlyearlierandmoresevereintheratswhichonlyreceivedLPSinjectionthanintheTGF-βtreatedrats.Thedurationoftheinflammationwasalsomuchlongerintheun-treatedratsthaninthetreatedrats(P<0.001).CONCLUSION:TGF-βpurifiedfromhumanplateletsmaypartlypreventthedevelopmentofEIUandefectivelyreducetheseverityofthein-flammationinducedbyLPSinjection.

关 键 词：转化生长因子 内毒素 葡萄膜炎 抑制作用 TGF-Β 

分 类 号：R773.905[医药卫生—眼科] R977.6[医药卫生—临床医学]