紫外诱变获得耐高浓度丙烯腈菌株的研究  

Study on ultroviolet mutagenesis to obtain strains with good acrylonitrile endurance

作　　者：孙晓君[1] 朱婷婷[1] 秦智[1] 蒋忠平[1] 魏金芝[1] 张艳[1] 薛滨泰[1] 

机构地区：[1]哈尔滨理工大学化学与环境工程学院,黑龙江哈尔滨150040

出　　处：《哈尔滨工业大学学报》2005年第10期1376-1378,1393,共4页Journal of Harbin Institute of Technology

基　　金：哈尔滨市科委攻关项目(2004AA4CS049);哈尔滨市青年科学研究基金资助项目(2004AFQXJ041)

摘　　要：为获得耐高浓度丙烯腈的菌株,利用紫外线对珊瑚诺卡氏菌进行诱变,并将诱变后生长情况较好的突变株在含一定浓度丙烯腈的培养基中进行筛选.结果表明:在紫外灯功率为20 W,照射距离为10 cm的条件下,诱变时间为3 m in和4 m in,致死率均大于90%;而诱变时间为5 m in,致死率为100%.诱变之后,从含低浓度丙烯腈的的培养基中,筛选出了9株有利突变的菌株.通过在含高浓度丙烯腈的培养基中复筛得到2株耐5.82%丙烯腈的突变株,其诱变时间为4 m in.Acrylamide with good quality and high purity can be produced by microbial method, which shows an excellent application future for its less environmental pollution. The production of acrylamide is affected directly by the endurable ability of strains to acrylonitrile. The purpose of the experiment was to get strains with good acrylonitrile endurance by mutagenizing the strains of Norcardia corailina with ultraviolet rays. The mutants in good condition were selected in the culture medium of certain concentration of acrylonitrile. The results showed that while the raying distance was 10 cm and an ultraviolet lamp with 20 W acted on the strain of Norcardia corailina 3 min and 4 min, the lethal ratio was high than 90% ; While the raying time was 5 min, the lethal ratio was 100%. In the culture medium of low concentration of acrylonitrile, 9 mutants which were in good condition were obtained. Through re-screen, 2 mutants which can endure 5.82% acrylonitrile were obtained. The mutagenesis time of the two mutants was 4 min.

关 键 词：珊瑚诺卡氏菌 紫外诱变 丙烯腈 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]