环氧合酶-2和p27^(kip1)蛋白在幽门螺旋杆菌感染胃癌变过程中的表达及其机制  

Expression of COX-2 and p27^(kip1) during the course of stomach cancerization after Hp infection

作　　者：刘登洋[1] 陈百芳[1] 曹华祥[2] 孟东[2] 狄长华[3] 

机构地区：[1]苏州大学附属第四医院无锡市第四人民医院消化科,江苏无锡214062 [2]苏州大学附属第四医院无锡市第四人民医院肿瘤外科,江苏无锡214062 [3]徐州医学院附属二院肿瘤外科

出　　处：《中华实验外科杂志》2005年第10期1193-1194,共2页Chinese Journal of Experimental Surgery

摘　　要：目的探讨幽门螺旋杆菌(Hp)感染胃癌变过程中不同病理组织类型的环氧合酶2(COX2)和p27kip1蛋白表达及其分子生物学机制。方法经胃镜和病理诊断明确的Hp感染患者的不同胃黏膜组织标本共200例。包括慢性浅表性胃炎(CSG)、肠上皮化生(IM)、不典型增生(AH)、胃癌(GCA)各50例,分别以ABC法和SP法测定COX2和p27kip1蛋白表达情况。结果COX2随癌变过程中阳性率和表达强度不断提高,分别为12%、42%、52%、74%,GCA组与其他组相比,差异有统计学意义(P<0.05)。而p27kip1随癌变过程,表达率逐步下降,分别为86%、46%、34%、26%。与其他组比较差异有统计学意义(P<0.05)。COX2和p27kip1表达呈显著负相关(r=-0.87,P<0.01)。结论COX2是Hp感染胃癌变过程的早期事件,并参与了胃癌变的全过程。COX2抑制了p27kip1的蛋白表达,两者负性相关。Objective To explore the molecular biological mechanism of cancerization in the different gastric pathologic tissue types after Hp infection by studying the expression of protein COX-2 and p27lupl. Methods 200 different samples of gastric mucous membrane were selected, which were taken from patients definitely infected with Hp by diagnosis of gastroscopy and pathology, including chronic superfical gastritis （CSG）, intestinal metaplasia （IM）, atypical hyperplasia （AH） and gastric carcinoma （GCA）, Each had 50 specimens. The methods of ABC or SP were used to detect the expression of protein COX-2 or p27^kip1. Results Along with the development of cancerization, the expression positive rate of COX-2 was increased gradually, which was 12%, 42%, 52% and 74% respectively. In addition, compared with other groups, the expression positive rate of COX-2 was significantly increased in group GCA （P 〈0.05）. But the expression positive rate of p27^kip1 was gradually decreased, which was 86 %, 46 %, 34 % and 26 % respectively with the difference being significant between any two groups （P 〈0.05）. The expression of of COX-2 was negatively correlated with that of p27^kip1（ r = -0.87, P 〈 0.01）. Conclusion COX-2 was the early event during the course of gastric cancerization after Hp infection and it took part in the total process. COX-2 inhibited the protein expression of p27^kip1 and they were in a negative correlation.

关 键 词：幽门螺旋杆菌 环氧合酶类 胃癌 P27^KIP1蛋白 幽门螺旋杆菌感染 环氧合酶-2 癌变过程 胃黏膜 幽门螺旋杆菌(Hp) P27^KIP1表达 

分 类 号：R735.2[医药卫生—肿瘤]