谷氨酰胺对阻塞性黄疸大鼠肝细胞凋亡和相关基因Bcl-2及Bax表达的影响  

Effects of glutamine on hepatocytes apoptosis,expressions of Bcl-2 and Bax in rats with obstructive jaundice

作　　者：程雷[1] 谭广[1] 王举[2] 石爱平[2] 谭毓铨[2] 王忠裕[1] 

机构地区：[1]大连医科大学第一附属医院普通外科,辽宁大连116011 [2]吉林大学第一医院普通外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第5期675-677,共3页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30300366)

摘　　要：目的:探讨谷氨酰胺对阻塞性黄疸大鼠肝细胞凋亡的影响。方法:健康雄性Wistar大鼠50只,随机分成空白对照组(SL,n=10)、阻塞性黄疸组(OJ,n=20)和谷氨酰胺治疗组(TG,n=20)。用原位末端标记法(TUNEL)和免疫组织化学方法检测50只大鼠肝脏组织细胞凋亡和凋亡相关基因Bcl-2及Bax蛋白的表达。结果:TG治疗2周时大鼠肝细胞Bcl-2蛋白的表达增加,强阳性表达();Bax的表达下降,中阳性表达();凋亡指数为15.75±3.68,与SL和OJ 2周组比较,差异具有显著性(P<0.05)。结论:谷氨酰胺能够降低阻塞性黄疸肝细胞凋亡,增加Bcl-2蛋白表达,降低Bax蛋白表达。Objective To explore the effects of glutamine on hepatocytes apoptosis in rats with obstructive jaundice. Methods 50 male Wistar rats were randomly divided into three groups., control （SL, n= 10）, obstructive jaundice group （O J, n = 20） and glutamine treatment group （TG, n = 20）. The hepatocytes apoptosis and the expressions of Bcl-2 and Bax were detected by using TUNEL and immunohistochemistry method. Results The expression of Bcl-2 in rat hepatocytes was increased 2 weeks after treatment in TG group. It expressed positively （＋＋＋）. The expression of Bax was also decreased compared with the SL and OJ groups in two weeks. It expressed positively （＋＋）. Apoptotic index was 15.75 4±3.68. The difference was significant （P〈0. 05）. Conclusion The glutamine can decrease hepatocytes apoptosis, the expression of Bax, and increase the expression of Bcl-2.

关 键 词：胆汁淤积/药物治疗 谷氮酰胺 肝细胞 细胞凋亡 

分 类 号：R575.7[医药卫生—消化系统]