丹参对肝癌致鼠梗阻性黄疸时VEGF表达的影响  

Effect of Danshen injection on VEGF expression in SD rats with malignant obstructive jaundice caused by hepatocellular carcinoma

作　　者：夏荣龙[1] 陈存平[1] 

机构地区：[1]华中科技大学同济医学院普外科,湖北武汉430030

出　　处：《第四军医大学学报》2005年第18期1673-1676,共4页Journal of the Fourth Military Medical University

基　　金：河南大学基金项目(XK03YBDYX16)

摘　　要：目的:建立恶性梗阻性黄疸模型,通过对模型鼠ip丹参注射液,观察其对肝癌瘤体大小、抑癌率及抑转移率和肝癌、癌周、临近肝叶及肺组织中血管内皮生长因子(VEGF)的表达,评价其作用.方法:用Walker256肝癌株近肝门部肝实质内种植致移植性肝癌侵袭高位胆管,造成胆道癌性狭窄,以建立SD大鼠恶性梗阻性黄疸模型.将模型鼠分成4组,通过对模型鼠腹腔内分别注射等量的生理盐水(n=24)、肌苷+VC(n=40)、丹参(n=40)和5FU(n=40).观察肝癌瘤体大小、抑癌率及抑转移率和肝癌、癌周、临近肝叶及肺组织中VEGF的表达.结果:丹参组与生理盐水组和肌苷+VC组相比,肝癌平均瘤体减小(0.5±0.2)cm3、抑癌率(41.7%)及抑转移率(59.7%,81.1%)增高(P<0.01);与5FU组相比,平均瘤体却较大(P<0.01).在抑癌率及抑肝转移率方面,丹参组除抑肺转移率外与5FU组相比无差异.丹参组VEGF在肝癌组织和癌周组织中的表达率分别低于生理盐水组(P<0.01)、肌苷+VC组(P<0.01)和5FU组(P<0.05).结论:丹参在肝癌致梗阻性黄疸时,通过促进肝癌细胞分化成熟、抑制肝癌细胞增殖、降低肝癌组织和癌周组织中VEGF表达,而对肿瘤发展起抑制作用.AIM： To establish malignant biliary obstruction model of SD rats and to study the effects of Danshen injection on the tumor size, inhibition rates and metastasis rates of hepatocellular carcinoma and the expression of vascular endothelia cell growth factor （VEGF） in carcinoma and peri-carcinoma tissues, adjacent lobe （left-internal lobe ） as well as lung tissues. METHODS： Malignant biliary obstruction models of SD rats were made by vaccination of transplanted tumor in liver near the porta hepatis with walker-256 hepatocarcinoma cell line, which infiltrated and metastasized to proximal common bile duct and caused its narrowness and obstruction. The SD rats were divided into 4 groups： 0.9% NS （ n =24 ）, Inosine ＋ VC （ n = 40）, Danshen （ n=40） and .5-FU （ n=40 ） respectively. The diameter of transplanted tumor, inhibition rates and metastasis rates of hepatocellular carcinoma and the expression of VEGF in carcinoma and peri-carcinoma tissues, adjacent lobe （ leftinternal lobe） as well as lung tissues were observed. Statistical analysis was made. RESULTS： The diameter of the tumor was reduced and the inhibition rates and metastasis rates of hepatocellular carcinoma were upgraded after treatment of Danshen injection compared with those in the rats treated by 0.9% NS. and Inosine combined with VC （ P 〈 0.01 ）, but the diameter of tumor was slightly downgraded after treatment of Danshen injection compared with that in the rats treated by 5-FU （ P 〈 0.01）. The inhibition rates （41. 7%） and the liver metastasis rate （59. 7% and 81.1% ） of hepatocellular carcinoma in rats treated by Danshen injection were similar with those in the rats treated by 5-FU （ P 〈 0. 01 ）. The expressions of VEGF in carcinoma and peri-carcinoma tissues were significantly downgraded after treatment of Danshen injection compared with those in the rats treated by 0.9% NS and Inosine combined with VC （ P〈0.01 ） and with those in the rats treated by 5-FU （ P〈 0. 05）. CONCLUSIO

关 键 词：黄疸 阻塞性 疾病模型 动物 肝肿瘤 Walker-256肝癌株 血管内皮生长因子类 丹参 

分 类 号：R735.5[医药卫生—肿瘤]