恒磁场对血管紧张素Ⅱ作用下血管平滑肌细胞VCAM-1分泌与表达的抑制作用  

Effects of constant magnetic field on secretion and expression of vascular cell adhesion molecule-1 in human vascular smooth muscle cells induced by angiotensin Ⅱ

作　　者：程何祥[1] 周廉[2] 王海昌[1] 郭文怡[1] 张荣庆[1] 李争显[2] 

机构地区：[1]西安第四军医大学西京医院心内科,710032 [2]西安西北有色金属研究院

出　　处：《解放军医学杂志》2005年第9期810-812,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题(编号30270396)

摘　　要：目的观察不同强度恒磁场对血管紧张素Ⅱ(AngⅡ)作用下人血管平滑肌细胞(VSMC)分泌与表达血管细胞黏附分子1(VCAM1)的影响。方法采用体外培养第4～6代的人脐动脉VSMC,实验分为6组,即对照组、AngⅡ(1×10-6mol/L)组及AngⅡ+不同磁感应强度(1、5、10、50Gs)的恒磁场组。各组细胞于培养及恒磁场作用12h后收集标本,ELISA法检测VCAM1的分泌量,免疫细胞化学法检测VCAM1的蛋白表达。结果AngⅡ刺激VSMC12h,VCAM1的分泌量与对照组比较显著增高(P<0.05);而5、10、50Gs恒磁场组细胞VCAM1的分泌量显著低于AngⅡ组(P<0.05)。结论5～50Gs的恒磁场可拮抗AngⅡ的作用,抑制VSMC分泌与表达VCAM1。Objective To investigate the effects of constant magnetic field on secretion and expression of vascular cell adhesion molecule-1 （VCAM-1） in human vascular smooth muscle cells （VSMCs） induced by angiotensin Ⅱ （AngⅡ）. Methods The fourth to sixth passages of in vitro cultured VSMCs from human umbilical artery were used. The cells were divided into six groups, i.e. control group, Ang Ⅱ （1）〈10^-6mol/L） group, Ang Ⅱ with exposure to 1, 5, 10 or 50 Gs of constant magnetic field groups. Samples were collected 12 hours after intervention. The secretion of VCAM-1 was assayed by enzyme linked immunosorbent assay （ELISA） and the expression of VCAM- 1 was assessed by immunocytochemistry. Results The secretion of VCAM-1 was increased significantly 12 hours after intervention with AngⅡ of 10^-6mol/L （P〈0. 05 vs control）. However, in 1, 5, 10 and 50 Gs groups, the secretion of VCAM-1 was significantly decreased compared with the Ang Ⅱ group （P〈0. 05）. Conclusion Five to fifty Gs of constant magnetic field can antagonize the effects of AngⅡon VSMCs, i.e. decrease the secretion and expression of VCAM-1 in VSMCs.

关 键 词：电磁场 血管紧张素Ⅱ 肌 平滑 血管 血管细胞粘附分子-1 

分 类 号：R363[医药卫生—病理学]