张应力对面颌肌细胞烟碱样乙酰胆碱受体基因表达影响的体外研究  

Nicotinic acetylcholine receptor (nAChR) expression in maxillofacial skeletal myocytes in response to strain in vitro

作　　者：高辉[1] 肖丹娜[2] 罗颂椒[3] 李小玉[4] 林珠[1] 

机构地区：[1]西安第四军医大学口腔医学院正畸科,710032 [2]天津市口腔医院正畸科 [3]成都四川大学华西口腔医院正畸科 [4]成都四川大学基础医学院细胞教研室

出　　处：《实用口腔医学杂志》2005年第5期625-628,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金(30171024)

摘　　要：目的评价张应力作用后面颌肌细胞烟碱样乙酰胆碱受体基因表达变化。方法采用已经建立的面颌部肌细胞体外力学刺激—细胞培养模型,半定量RT-PCR技术检测nAChRα1亚基基因加力前后转录水平变化。结果未发现有明显统计学差异。结论单纯张应力对体外培养小鼠颌面部骨骼肌细胞nAChR基因表达影响较小。Objective：To elucidate the effect of strain on nicotinic acetylcholine receptor （nAChR） mRNA expression in maxillofacial skeletal myocytes. Methods ： Skeletal myocytes of BALB/C mice were cultured and strained by a straining device, Focel, with the force of 0.1 % cell deformity for 2,4,6,8 and 12 h respectively, Then the cells were collected,nAChRα1 subunit mRNA expression was examined by Semi-quantitative RT-PCR test. Results：No significant difference was found in nAChtRα1 subunit mRNA expression between strained and unstrained cells（ P =0, 30） , no difference was found among the cells treated for different times（ P = 0.13 ）. Conclusion：Strain at the force of 0. 1% cell deformity has no effect on the nicotinic acetylcholine receptor （nAChR） expression in maxillofacial skeletal myoeytes of mouse in in vitro.

关 键 词：骨骼肌细胞 应力 乙酰胆碱受体 

分 类 号：R783.5[医药卫生—口腔医学]