解偶联蛋白3基因启动子区-55(C>T)多态与中国人静息能量消耗及体脂含量与分布的关系(英文)  

Effect of polymorphism of uncoupling protein 3 gene -55 (C>T) on the resting energy expenditure, total body fat and regional body fat in Chinese

作　　者：方启晨[1] 贾伟平[1] 杨明[1] 包玉倩[1] 陈蕾[1] 张蓉[1] 项坤三[1] 

机构地区：[1]上海市糖尿病研究所上海交通大学附属第六人民医院内分泌代谢科,200233

出　　处：《中华医学遗传学杂志》2005年第5期485-488,共4页Chinese Journal of Medical Genetics

基　　金：上海科技发展基金项目(99XD14017) ;上海市卫生系统百名跨世纪优秀学科带头人培养计划项目(97BR039)~~

摘　　要：目的研究解偶联蛋白3基因(UCP3)启动子区-55(C>T)多态与中国人静息能量消耗、体脂参数的关系。方法在300名中国人(正常体重91人,超重/肥胖209人)中,用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restrictionfragment length polymorphisms ,PCR-RFLP)检测UCP3基因启动子区-55(C>T)变异,并测定其静息能量消耗、体脂含量及分布。结果UCP3基因启动子区-55(C>T)多态基因型频率与肥胖及肥胖类型均无相关。正常体重组TT基因型者静息能量消耗水平显著高于CT及CC基因型者(P<0·05) ;超重/肥胖组各基因型者间比较亦有同样趋势。在超重/肥胖组,TT基因型者FM/FFM值与CT及CC基因型者差异有显著意义(P<0·01)。结论UCP3基因启动子区-55(C>T)多态与中国人静息能量消耗相关,该变异可能通过对静息能量消耗的影响调节机体的能量代谢。Objective To investigate the relationship of the C to T variant at the - 55 site of the promoter region of uncoupling protein 3 gene （UCP3） with the resting energy expenditure and the parameters of body fat in Chinese population. Methods Three hundred Chinese （91 normal weight subjects, 209 overweight/obesity subjects） were genotyped for the UCP3 gene - 55 （ C 〉 T） by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Resting energy expenditure （REE）, fat mass （FM）, fat free mass （FFM） and the parameters for regional adipose tissue distribution were measured. Results Genotype frequencies of UCP3 gene - 55 （ C 〉 T） were not associated with obesity and different types of obesity. The REE level in normal weight subjects with TT homozygotes was higher than that in those with CT hetemzygotes and CC homozygotes （ P = 0.0200）. Similar tendency was also observed in overweight/obesity subjects. The FM/FFM exhibited significant differentce between the overweight/obesity subjects with a TT genotype and those with a CTor CC genotype （P =0.0096）. Cortdusion The level of difference in REE caused by the polymorphism of promoter region of UCP3 - 55（C 〉 T） may play a role in energy metabolism in Chinese.

关 键 词：解偶联蛋白3基因启动子区-55 基因多态性 中国 静息能量消耗 体脂含量 肥胖 

分 类 号：R394[医药卫生—医学遗传学]