家兔“风门”穴肌膜神经末梢感受器通路的实验  

Experiment of the receptor closed-circuit of nerve terminal of sarcolemma at Fengmen point in rabbits

作　　者：徐志强[1] 章云海[1] 卢文[2] 李玉[3] 李静[1] 曾因明[4] 刘克芳[1] 陆爱蓉[1] 耿祝生[1] 雷玲[1] 沈国容[1] 李小明[1] 赵琳[1] 

机构地区：[1]连云港市第一人民医院麻醉科,江苏省连云港市222002 [2]连云港市第一人民医院针灸科,江苏省连云港市222002 [3]连云港市第一人民医院肿瘤生物实验室,江苏省连云港市222002 [4]江苏省麻醉学重点实验室,江苏省徐州市221002

出　　处：《中国临床康复》2005年第33期36-38,i0001,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(30271260);江苏高校省级重点实验室第三批对外开放课题(K2093)~~

摘　　要：目的:观察家兔“风门”穴肌膜神经末梢感受器通路与皮肤末梢感受器通路的关系。方法:实验于2004-10/11在徐州医学院附属连云港医院肿瘤生物实验室完成。在4只雄性家兔经“风门”穴的肌膜注射神经追踪剂荧光素核黄,成活18～24h,分别取C2～L5背根神经节、颈、胸交感神经节、肠系膜下神经节、大脑、小脑、丘脑、脑干、脊髓、胃、小肠、心肌、肺、骶部骶棘肌、肝、膀胱、脾、腹主动脉、肠系膜下动脉、腹腔淋巴节、牵涉区部位的皮、骶部的皮及耳,冰冻连续切片,分别做成两套,一套蒸馏水贴片,直接在OlympusZX-004荧光显微镜下观察,另一套苏木精-伊红染色后在荧光显微镜下观察。观察其是否与皮内感受器通路相关。结果:4只家兔均成功地进行了风门穴肌膜注射荧光素核黄,共获得胶片288张。①在C3～L3脊神经节发现标记细胞,以T3～T12标记细胞较多;在颈、胸交感神经节及肠系膜下神经节发现标记细胞;在胃黏膜下标记到副交感神经元;在小肠黏膜及黏膜下标记到大量的节细胞;在大脑的侧脑室壁标记到大量带状排列的小细胞;下丘脑的四脑室底部标记到部分多角细胞。②在脊髓前、后角、小脑、骶棘肌、背阔肌、膈肌的肌膜、心肌的内、外膜,胃、膀胱壁、肺支气管壁、肝管壁、胆囊壁的外膜和黏膜、肩关节滑膜、腹主动脉、肠系膜动脉壁的内、外膜、以及牵涉区和骶部及耳皮内毛细血管袢发现有荧光密集区。结论:家兔“风门”穴肌膜神经末梢感受器通路网络由大脑和下丘脑高位中枢的部分神经细胞、脊神经节、交感神经节及节后神经元、副交感神经元等四大神经网络组成,较皮肤末梢感受器通路网络更为广大。AIM： To observe the relationship of the receptor closed-circuit of nerve terminal of sarcolemma at Fengmen （UB 12） point in rabbits and receptor closed-circuit of the skin end-brush. METHODS： The experiment was done in the Biochemical Laboratory of Tumor, Affiliated Lianyungang Hospital, Xuzhou Medical College from October to November 2004. The sarcolemrna of the four male rabbits was treated with the nerve tracer fluorescein at Fengmen point, surviving at 18 to 24 hours, the derrn of C2-L5 dorsal root ganglions, sympathetic ganglion of neck and chest, inferior mesenteric ganglion, cerebrum, cerebellum, thalamus, brain stem, spinal cord, stomach, small intestine, myocardium, lung, sacrospinal muscle, liver, bladder, spleen, abdominal aorta, inferior mesenteric artery, lymph node at abdominal cavity and the part of involved regions and the derm at sacral region, which were froze and made into serial sections of two sets. One set was distilled water sticking section, and observed under the Olympus ZX-004 fluorescence microscope directly. The other set was observed under the fluorescence microscope after hematoxylin-eosin staining. To observe if it had relationship with the skin receptor closed-circuit. RESULTS： Totally 4 rabbits were treated with sarcolemma injection with fluorescein at Fengmen points successfully, and totally 288 pieces of the films were gained. ① The labeling cells were found at C3-Ls spinal ganglia, and most of the labeling cells appeared at T3-T12; The labeling cells were also found at the sympathetic ganglion of the neck and chest and inferior mesenteric ganglion; The .parasympathetic neurons labeled at stomach mucous membrane; A large number of ganglion cells labeled at small intestine mucous membrane and mucous membrane; A great amount of mini cells which arranged bandlike labeled at the cerebral lateral ventricle wall; Part of multi-angle cells labeled at the bottom of the epithalamic tetra-brain ventricle.② There were fluorescence dense regions found at anterior spinal cord,

关 键 词：风门 肌膜 神经节 脊 神经节 交感 神经元 下丘脑 

分 类 号：R745[医药卫生—神经病学与精神病学]