神经生长因子对缺氧/缺糖诱导的大鼠小脑皮质神经细胞凋亡的影响  

Effects of nerve growth factor on neural apoptosis of cerebellar cortex induced by hypoxia / hypoglycemia in rats

作　　者：李强[1] 赵宝东[2] 王冬阳[1] 

机构地区：[1]山东省交通医院神经内科,山东省济南市250031 [2]锦州医学院解剖教研室,辽宁省锦州市121001

出　　处：《中国临床康复》2005年第33期61-63,F0003,共4页Chinese Journal of Clinical Rehabilitation

基　　金：辽宁省教育委员会重点科研课题(991721597)~~

摘　　要：目的:观察神经生长因子对缺氧/缺糖诱导的原代培养的大鼠乳鼠小脑皮质神经细胞凋亡的保护作用。方法:实验于2003-03/10在锦州医学院科技实验中心细胞培养实验室进行。将原代培养7d的大鼠乳鼠小脑皮质细胞分为5组:①正常对照组:不干预。②模型组:换含0.5mmol/L连二亚硫酸钠的无糖Earles液继续培养48h,建立了缺氧/缺糖诱导的神经细胞凋亡模型。③神经生长因子5,50,100μg/L组:提前24h加入5,50,100μg/L的神经生长因子,同前造模。通过光学显微镜观察各组细胞损害情况,流式细胞仪检测细胞凋亡率。结果:①细胞凋亡率:模型组和神经生长因子5μg/L组高于正常对照组([47.08±13.27)%,(39.01±11.08)%,(4.08±2.45)%,P<0.01]。神经生长因子50,100μg/L组低于模型组和神经生长因子5μg/L组([15.09±5.68)%,(10.56±4.72)%,P<0.01]。②细胞形态:模型组TUNEL染色发现大量阳性细胞,细胞形态亦有明显损伤变化。神经生长因子50,100μg/L组细胞形态结构损伤减轻或基本恢复正常。结论:给予外源性神经生长因子,能够抑制缺氧/缺糖造成的小脑皮质神经细胞凋亡,对缺氧/缺糖引起的小脑皮质神经细胞损伤有明显保护作用。AIM：To observe the protective effects of nerve growth factor （NGF） on neural apoptosis induced by oxygen/hypoglycemia in rats aged 1-3 days with primary culture. METHODS： The experiment was done in the laboratory of cell culture, Experimental Center of Science and Technology, Jinzhou Medical College between March and October ：2003. The cerebellar cortex of rats aged 1-3 days after primary culture for 7 days were divided into 5 groups： ① Normal control group： No intervention. ② Model group： The rats were cultured with Earle＇s liquor without sugar containing 0.5 mmol/L sodium dihionite continuously for 48 hours. The apoptosis models induced by hypoxia/hypoglycemia were built. ③ 5, 50, 100 μg/L NGF group： Twenty-four hours ahead of schedule, 5, 50, 100μg/L NGF were added to build models as before. The condition of cell lesion in every group was observed with light microscope, and the apoptosis rate was detected with the flow cytometer.RESULTS：① Apoptosis rate： It was higher in the model group and 5μg/L NGF group than that in the normal control group [（47.08 ± 13.27 ） %, （ 39.01 ± 11.08 ） %, （ 4.08±2.45 ） %, P 〈 0.01 ]. It was lower in the 50, 100μg/L NGF group than that in the model group and 5μg/L NGF group [（ 15.09±5.68 ）%, （ 10.56±4.72 ）%, P 〈 0.011. ② Formation of cells： A large number of positive cells were found in the model group with TUNEL staining, and there were also significant changes of injury in the formation of cells. The formation and structure of cells in the 50, 100μg/L NGF group had alleviative damage or recovery to normal basically. CONCLUSION： The administration of exogenous NGF can inhibit neural apoptosis of cerebellar cortex induced by hypoxia/hypoglycemia, which has significantly protective effects on injury of nerve cells of cerebellar cortex due to hypoxia/hypoglycemia.

关 键 词：脑缺氧 脑缺血 神经生长因子 小脑 细胞凋亡 

分 类 号：R745[医药卫生—神经病学与精神病学]