针刺对实验性坐骨神经根压迫模型大鼠脑组织中单胺递质的影响  

Effects of acupuncture on monoamine neurotransmitters in brain tissue of experimental rat models of sciatic nerve compression

作　　者：王胜 孙忠人[2] 佟帅[3] 单丽莉[2] 张晓雷 

机构地区：[1]解放军第二五五医院中医康复中心,河北省唐山市063000 [2]黑龙江中医药大学附属第二医院神经内科,黑龙江省哈尔滨市150001 [3]北京市海淀医院神经内科,北京市100080

出　　处：《中国临床康复》2005年第33期94-96,共3页Chinese Journal of Clinical Rehabilitation

基　　金：黑龙江省教育厅骨干教师资助项目(黑教科计划字2001010号)~~

摘　　要：目的:观察针刺对坐骨神经根压迫模型大鼠脑组织中单胺递质水平的影响,探讨针刺对镇痛、促进神经损伤再生修复的作用。方法:实验于2001-03/07在黑龙江中医药大学针灸生理实验室完成。选择Wistar大鼠90只,分为5组,电针治疗组、电针对照组、手针对照组、模型对照组及正常对照组各18只。前4组大鼠均进行坐骨神经根压迫模型制备。①电针治疗组:造模后第3天开始电针治疗,1次/d,每次15min。取穴:双侧L4,L6“夹脊”,患侧“环跳”、“阳陵泉”。进针深度约1cm,轻提插捻转后,针柄接电疗仪。连续波,频率2Hz,电流强度以大鼠腰肌及后肢轻度抖动为度。②电针对照组:取穴:双侧L4～6“夹脊”。余同电针治疗组。③手针对照组:取穴:双侧“肾俞”,患侧“环跳”、“阳陵泉”。采用轻提插捻转手法,不接电疗仪,间隔5min捻针1次,余同电针治疗组。④模型对照组:于造模后第3天开始,每日抓取1次,不做针刺等处置。⑤正常对照组:正常饲养,不做任何处置。各组分别于术后第7,14,28天各麻醉后处死6只大鼠,制备组织匀浆,离心后取上清液测定脑组织中5-羟色胺、5-羟吲哚乙酸及去甲肾上腺素含量应用荧光分光光度法。结果:90只大鼠全部进入结果分析,无脱失。①不同时间点各组大鼠脑组织中5-羟色胺水平的变化:造模后7d,电针治疗组和电针对照组大鼠脑组织中5-羟色胺水平显著高于正常对照组[(190.23±5.05),(170.80±6.99),(150.76±6.65)ng/g(P<0.01,<0.05)]。造模后14d,两电针组5-羟色胺水平进一步提高。至造模后28d,两电针组5-羟色胺水平下降,电针治疗组已接近正常水平(162.51±7.67)ng/g。②不同时间点各组大鼠脑组织中5-羟吲哚乙酸水平的变化:各组大鼠脑组织5-羟吲哚乙酸水平的差异均无显著性意义(P>0.05)。③不同时间点各组大鼠脑组织中去甲肾上腺素水平的变化:造模后7d及14d模型对照�AIM： To observe the influence of acupuncture on the level of monoamine neurotransmitters in brain tissue of rat models of sciatic nerve compression, and probe into the effect of acupuncture on analgesia and in promoting the regeneration and repair of nerve injury. METHODS： The experiment was carried out in the acupuncture physiological laboratorv of Heilongjiang University c,f Traditional Chinese Medicine between March and July 2001. Ninety Wistar rats were randomly divided into 5 groups with 18 rats in each group： electroacupuneture treated group, electroacupuncture control group, hand acupuncture control group, model euntrol group and nonnal cnntrol group. Rats in the first 4 groups were made into models of sciatic nerve compression. ① Electroaeupuncture treated group： The rats were treated with electmaeupunelure from the 3Tnd day after model establishment, once a day, 15 minutes for each time. Bilateral L4 and L6 Jiaji acupoiuts, ipsilateral Huantioo and Yanglingquart acupoints were selected. The needle was inserted for about 1 cm, and then the needle handle was connected to the electro-therapeutic apparatus after lifting, thrusting and twirling of needle. Continuous waves were given with the frequency of 2 Hz, the current intensity was judged with the mihl shaking of psoas muscle and hindlimbs of the rats. ② Electroacupuncture control group： Bilateral L4 and L6 Jiaji acupoints were selected, and other treatments were the same as those in the electroacupuncture treated group. ③ Hand acupuncture control group： Bilateral Shenshu aeupoints, ipsilateral Huantiao and Yanglingquan acupoints were selected, the lifting, thrusting and twirling of needle wee used without connecting with eleetrn-therapeutic apparatus, the needle was twirled once every 5 minutes, other treatments were the same as those in tile eleetroaeupuncture treated group. ④ Model control group： The rats were grasped once a day from the 3^nd day after model establishment, and they received no acupuncture treatmcnt. ⑤ Normal eo

关 键 词：坐骨神经/损伤 针灸效应 生物源单胺类 

分 类 号：R745.4[医药卫生—神经病学与精神病学]