加减二仙汤对急性不完全脑缺血大鼠脑水肿的影响  

Effect of modified erxian tang on brain edema in rats with incompletely cerebral ischemia

作　　者：高慧英[1] 戴兴歧 王洪海[3] 梁瑞兰[3] 李亚鲁[1] 

机构地区：[1]泰山医学院组织胚胎学教研室,山东省泰安市271000 [2]泰安第四医院中医科,山东省泰安市271000 [3]泰山医学院中医学教研室,山东省泰安市271000

出　　处：《中国临床康复》2005年第33期107-109,共3页Chinese Journal of Clinical Rehabilitation

基　　金：山东省教育委员会资助项目(J96K21)~~

摘　　要：目的:探讨加减二仙汤对急性不完全脑缺血再灌注大鼠脑组织含水量和超氧化物歧化酶活性的变化及缺血再灌不同时间脑组织的形态学改变。方法:实验于2002-12在泰山医学院形态学实验室完成。①选择99只中老年Wistar大鼠,随机分成假手术组11只、再灌对照组44只和再灌实验组44只。其中后两组又分为再灌6,24h,3,7d组,每组11只。②制备急性不完全脑缺血再灌模型,再灌实验组大鼠造模前给予加减二仙汤煎剂(由仙茅,仙灵脾,当归,黄芪,丹参,红花等中药组成,由泰山医学院附属医院中药房提供,每毫升含生药量14mg),假手术组和再灌对照组灌胃等量生理盐水。③各组于观测时间点取脑组织检测含水量和超氧化物歧化酶活性,并进行形态学研究。结果:99只大鼠均进入结果分析。①再灌实验6,24h,3d组脑含水量明显低于再灌对照6,24h,3d组[6h:(79.49±2.29),(81.04±0.09)%,t=2.374,P<0.05;24h:(78.97±2.84),(81.92±0.95)%,t=3.257,P<0.01;3d:(76.77±0.51),(79.88±0.76)%,t=4.837,P<0.01]。②再灌实验6,24h,3d组超氧化物歧化酶活性明显高于再灌对照6,24h,3d组[6h:(122.41±8.85),(113.40±9.00)μg/g,t=4.471,P<0.01;24h:(123.40±10.24),(118.54±4.16)μg/g,t=2.451,P<0.05;3d:(131.36±5.08),(127.74±3.38)μg/g,t=2.963,P<0.05]。③形态学研究结果表明,再灌对照组脑组织损伤严重,主要表现为顶叶皮质毛细血管内液体渗出,管腔狭窄甚闭锁,神经细胞损伤、皱缩甚者脱失;而再灌实验组损伤明显减轻。结论:给予加减二仙汤大鼠脑组织含水量降低,超氧化物歧化酶活性升高,说明加减二仙汤可减轻脑水肿产生,抑制再灌注损伤,保护脑组织。Abstract AIM： To explore the effects of modified erxian tang on water content, superoxide dismutase （SOD） activity and morphological changes in brain tissue of rats with acute incompletely cerebral ischemia reperfusion injury. METHODS： The experiment was conducted in the laboratory of Morphology, Taishan Medical College in December 2002. ① Ninety middle-age and old Wistar rats were selected and randomized into sham operation group （n=11）, control group （n=44） and experimental group （n= 44）. The latter two groups were subdivided into 6-hour, 24-hour, 3-day and 7-days groups with 11 rats in each group. ② Before rat models of acute incompletely cerebral ischemia reperfusion were made, the experimental group was given modified erxian tang （consisting of common curculigo rhizome, xianlingpi, chinese angelica, membranous milkvetch root, danshen root, safflower, supported by the Dispensary of Traditional Chinese Medicine, the Affiliated Hospital of Taishan Medical College, with 14 nag crude drug per milliliter）, and the control and sham operation groups were given the same volume of normal saline via stomach.③ Brain tissues in each group were taken out at different time points to detect water content and SOD activity, and then the morphological observation was performed. RESULTS： All the 99 rats were involved in the result analysis. Compared with the 6-hour, 24-hour and 3-day control group, the water content in the 6-hour, 24-hour and 3-day experimental group was significantly declined[hour 6： （ 79.49±2.29 ） ,（81.04±0.09）%,t =2.374,P ≤ 0.05 ;hour 24 ： （78.97±2.84）, （81.92 ±0.95）% ,t =3.257,P 〈 0.01;day 3：（76.77 ±0.51 ）, （79.88 ± 0.76）% ,t =4.837,P 〈 0.01], while the SOD activity was increased obviously [hour 6 ： （122.41±8.85）,（113.40±9.00） NU/mg, t=4.471 ,P 〈 0.01 ;hour 24： （123.40± 10.24）,（118.54±4.16） NU/mg, t=2.451,P 〈 0.05 ;day 3 ：（131.36± 5.08）,（127.74±3.38） NU/mg, t=2.963,P 〈 0.05]. Morphological stu

关 键 词：脑缺血 脑水肘 二仙汤 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]