离体海马脑片CA1区锥体细胞缺氧期间电生理指标变化与N-甲基-D-天冬氨酸受体阻断剂MK801干预后的变化(英文)  

Effects of the blocker of N-methyl-D-aspartate receptor MK801 on the electrophysiological changes of CA1 neurons during hypoxia in isolated hippocampal slices of rats

作　　者：张育才[1] 曾因明[2] 王钧 

机构地区：[1]解放军第九七医院麻醉科,江苏省徐州市221004 [2]徐州医学院江苏省麻醉学重点实验室,江苏省徐州市221002 [3]Healthy-Science Center,State University of New York,New York,USA

出　　处：《中国临床康复》2005年第33期146-148,共3页Chinese Journal of Clinical Rehabilitation

基　　金：江苏省教育厅资助课题(KJS04004)~~

摘　　要：背景:海马CA1区锥体细胞是对缺血缺氧性损伤最为敏感的神经元,缺血缺氧后海马CA1区锥体细胞膜电位表现为缺氧早期细胞膜的超极化,随着缺氧时间的延长,细胞膜发生缓慢去极化及快速去极化,引起神经元不可逆性损伤。目的:应用细胞内记录技术,观察N-甲基-D-天冬氨酸受体阻断剂MK801对离体海马脑片CA1区锥体细胞缺氧期间电生理指标的变化。设计:观察对比实验。单位:解放军第九七医院,徐州医学院江苏省麻醉学重点实验室,Healthy-ScienceCenter,StateUniversityofNewYork。材料:实验于2002-09/2003-02在美国纽约州立大学医学中心完成。选择成年雄性SD大鼠5只,预吸纯氧3min后以体积分数为0.02的异氟醚麻醉,快速断头取脑,制备离体海马脑片。方法:大鼠海马脑片随机分为单纯缺氧组和MK801组,每组10个。单纯缺氧组海马脑片给予10min缺氧;而MK801组的海马脑片在缺氧前10min及10min的缺氧期间分别应用100μmol/L的MK801。所有脑片均给予60min的复氧。应用细胞内记录技术记录海马CA1区神经元缓慢去极化及快速去极化的时间、快速去极化的幅度。在复氧末,应用细胞内注入电流及经Schaffer通路刺激,观察神经元对刺激的反应。主要观察指标:①两组海马CA1区锥体神经元缓慢去极化速率。②两组海马CA1区神经元的快速去极化时间。③两组海马CA1区神经元的快速去极化幅度。④MK801对海马CA1区神经元功能恢复的影响。结果:①海马CA1区锥体神经元缓慢去极化速率:单纯缺氧组显著高于MK801组[(0.20±0.05)mV/s,(0.08±0.03)mV/s,(P<0.05)]。②海马CA1区神经元的快速去极化时间:MK801组显著高于单纯缺氧组[(537±139)s,(261±26)s,(P<0.05)]。③海马CA1区神经元的快速去极化的幅度:MK801组显著小于单纯缺氧组[(4±13)mV,(53±7)mV,(P<0.05)。④在复氧末期,MK801组的10个神经元中,9个神经元恢复对刺激的反应。结论:N-甲基-D-�BACKGROUND： Pyramidal cells in hippocampal CA1 region are neurons most susceptible to ischemia-hypoxia damage. Their membrane potential is shown as hyperpolarization of cell membrane during early hypoxia. With the progress of hypoxia time, cell membrane has slow and rapid hyperpolarization, which causes irreversible damage to neurons. OBJECTIVE： To investigate the effects of the blocker of N-methyl-D-as- partate receptor MK801 on the electrophysiological changes of CA1 neurons during hypoxia in isolated hippocampal slices of rats with intracellular recording technique.DESIGN： Observational and controlled study. SETTING： The 97^th Hospital of the Chinese PLA, Provincial Key Anesthesiology Laboratory of Xuzhou Medical College; Center of Health Science, State University of New York. MATERIALS： The experiment was conducted from September 2002 to March 2003 in the State University of New York. Five adult male SD rats were anesthetized with 0.02 volume of isoflurane after 3 minutes＇ pre-oxygenation with oxygen. METHODS： The hippocampal slices from the rats were randomly divided into simple anoxia group （n=10） and MK801 group （n=10）. The slices in simple anoxia group were only subjected to 10-minute hypoxia with the artificial cerebrospinal fluid （ACSF）, and the slices in MK801 group were treated with 100μmol/L MK801 for 10 minutes before and during 10 minutes of hypoxia. The neuronal membrane potential before hypoxia, the rate of slow depolarization, the amplitude of and time to rapid depolarization were recorded with intracellular recording technique described in the literature. Meanwhile, the neuronal response to the intracellular current injection and Schaffer collateral stimulation were observed respectively at the end of 60 minutes＇ re-oxygenatlon. MAIN OUTCOME MEASURES： ① The rate of slow depolarization;② the time to rapid depolarization;③ the amplitude of rapid depolarization;④ the effect of MK801 on the functional recovery of neurons. RESULTS： ① The rate of slow depola

关 键 词：脑 受体 N-甲基-D-天冬氨酸/拮抗剂和抑制剂 细胞低氧 海马 电生理学 

分 类 号：R741.044[医药卫生—神经病学与精神病学]