中风安口服液对动脉血栓形成和血液凝固的干预效应(英文)  

Effect of oral zhongfeng an liquid on arterial thrombosis and blood coagulation

作　　者：黎燕峰[1] 张永健[1] 王素敏[1] 郭鸣放[1] 宋建徽[1] 王永利[1] 

机构地区：[1]河北医科大学基础医学院药理教研室,河北省石家庄市050017

出　　处：《中国临床康复》2005年第33期156-158,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:中风安口服液是治疗脑血管疾病的新型中药制剂,主要成分为黄芪和水蛭,其中黄芪具有明显的益气活血作用,水蛭具有很强的抗血小板、抗血栓、缓解动脉痉挛和改善组织缺氧的作用。目的:观察中风安口服液对大鼠动脉血栓形成和对小鼠血液凝固及耐力的影响。设计:完全随机对照实验。单位:河北医科大学基础医学院药理教研室。材料:实验于2001-09/2002-04在河北医科大学药理研究室完成。药物影响血栓形成实验:第1组实验取Wistar大鼠40只,随机分为中风安3.0,6.0,12.0g/kg剂量组、阿司匹林0.3g/kg组和对照组,每组8只。第2组实验取Wistar大鼠50只,随机分为中风安3.0,6.0g/kg剂量组、脑血康3.0,6.0g/kg剂量组和对照组,每组10只。小鼠凝血时间实验:取小鼠50只,随机分为中风安6.0,12.0,24.0g/kg剂量组、阿司匹林0.3g/kg组和对照组,每组10只。小鼠游泳耗竭时间测定:取小鼠90只,随机分为中风安6.0,24.0g/kg剂量组;脑血康6.0,24.0g/kg剂量组,苯丙胺0.2g/kg组和对照组,每组15只。方法:药物影响血栓形成实验,第1组于术前24h及1h各组大鼠分别灌胃给予中风安(3,6,12g/kg),阿司匹林(0.3g/kg)和水。第2组大鼠术前24h及1h分别灌胃给予中风安(3,6g/kg)脑血康(3,6g/kg)和水。给药后腹腔注射氯胺酮50mg/kg麻醉,采用线栓血栓法,测定各鼠血栓湿重,比较各组血栓形成的差异。小鼠凝血时间的测定,分别灌胃给予中风安(24.0,12.0,6.0g/kg),阿司匹林(0.3g/kg)和水,于灌胃后1h用玻片法观察小鼠凝血时间。小鼠游泳耗竭时间的测定,灌胃给予中风安(6.0,24.0g/kg)和脑血康(6.0,24.0g/kg)及苯丙胺(0.2g/kg,)和水。1次/d,连续灌胃5d,于第5天给药后1h测定小鼠游泳耗竭时间。计算各组小鼠游泳耗竭时间的平均值。主要观察指标:①各组大鼠血栓形成抑制率。②各组小鼠凝血时间。③各组小鼠游泳耗竭时间。结果:参加实验的90只大鼠�OBJECTIVE： Oral zhongfeng an liquid is a new dose form of traditional Chinese medicine fur treating cerebrowlscular diseases. Its main components are astragalus and hirudo, the former is of obvious effects of replenishing qi and activating blood, and the latter is of stronger effects of antiplatelet, antithron,bosis and arteriospasm-reducing, as well as improving tissue anoxia. OBJECTIVE： To investigate the effects of oral zhongfeng an liquid on arterial thrombosis in rats, and blood coagulation and tolerance in mice. DESIGN： A completely randomized and controlled trial. SETTING： Pharnmcological Division of Basic Medical College, Hebei Medical University. MATERIALS： The experiment was completed from September 2001 to April 2002 at the Pharmacological Division of Basic Medical College, Hebei Medical University. The experiment of effect of the drug on thrombosis in rats： In the first study, totally 40 Wistar rats were at random divided into five groups： zhungfeng on liquid 3.0, 6.0, 12.0 g/kg, aspirin 0.3 g/kg and control group, with 8 in each group. In the second study, totally 50 Wistar rats were also at random divided into five groups： zhongfeng an liquid 3.0 and 6.0 g/kg, mu）xue kang 3.0 and 6.0 g/kg and control group, with 10 in each group. Clotting time study in mice： Totally 50 mice were randondy divided as ztumgfeng an liquid 6.0, 12.0, 24.0 g/kg, aspirin 0.3 g/kg and control group, with 10 in each group. Measurement of swimming exhaustion time of mice： Totally 90 mice were randomly divided as zhongfeng an liquid 6.0 and 24.0 g/kg, naoxue kang 6.0 and 24.0 g/kg, benzedrine 0.2 g/kg and control group, with 15 in each group. METHODS： In the experiment of effect of the drug on thrombosis： For the first study, 24 hours and 1 hour before operation the rats in all groups were respectively hy garage given oral zhongfeng on liquid （3, 6, 12 g/kg）, aspirin （0.3 g/kg）, and water. For the second study, 24 hours and 1 hour before operation the rats were respectively by garage given ora

关 键 词：血栓形成/中药疗法 水蛭 黄苠 大鼠 小鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]