复方缬芎提取物改善脑微循环障碍作用(英文)  

Improvement of valerian-ligusticum extract on cerebral microcirculatory disturbance

作　　者：薛存宽[1] 何学斌[1] 屈文[2] 李颖[1] 曾伶[1] 彭仁 

机构地区：[1]华中科技大学同济医学院老年医药学研究所,湖北省武汉市430077 [2]武汉大学医学部药学系,湖北省武汉市430071

出　　处：《中国临床康复》2005年第33期171-174,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:复方缬芎提取物中缬草提取物为天然的γ-氨基丁酸受体激动剂,可以松弛脑血管痉挛;川芎提取物通过血脑屏障并增进组织微循环、抑制血小板聚集及5-羟色胺释放。目的:探讨由有效组分配制成的复方缬芎提取物对脑缺血损害的防治效果。设计:完全随机、阴性和阳性对照实验。单位:华中科技大学同济医学院老年医药学研究所。材料:实验于2003-03/2004-05在华中科技大学同济医学院老年医药学研究所完成。动物:①脑组织营养血流灌注实验:选择昆明种小鼠50只,随机分为正常组、溶剂对照(模型)组、川芎嗪50mg/kg组、复方缬芎170mg/kg组及复方缬芎85mg/kg组,每组10只。②动-静脉旁路法血栓形成实验:选择Wistar大鼠50只,随机分为溶剂对照(模型)组、复方丹参5g/kg组、复方缬芎156mg/kg组、复方缬芎94mg/kg组及复方缬芎31.3mg/kg组,每组10只。③大脑中动脉阻断法实验:选择Wistar大鼠60只,随机分为假手术组、溶剂对照(模型)组、川芎嗪10mg/kg组、复方缬芎156mg/kg组、复方缬芎94mg/kg组及复方缬芎31.3mg/kg组,每组10只。④急性广泛性脑缺血实验:选择昆明种小鼠50只,随机分为正常组,溶剂对照(模型)组、川芎嗪10mg/kg组、复方缬芎200mg/kg组及复方缬芎40mg/kg组,每组10只。方法:①脑组织营养血流灌注实验中各组小鼠分别预先腹腔注射复方缬芎(85,170mg/kg)、川芎嗪(50mg/kg)或等容积助溶剂(0.2mL),20min后静脉注射垂体后叶素(2.5u/kg),10min后尾静脉注射同位素99Tcm+亚锡双半胱氨乙酯3.7×1010Bq/L(0.1mL/只)。注射15min后用放射免疫计数仪计取血和大脑组织脉冲强度。②动-静脉旁路法血栓形成实验,术前分别灌胃给复方缬芎156,94,31.3mg/(kg·d),复方丹参注射液[相当生药5g/kg·d)]和生理盐水,连续给药7d,1次/d,停药24h后戊巴比妥钠腹腔麻醉下以体外导流管(内置手术丝线)连接颈总静脉与颈总动脉。体外导�BACKGROUND： In valerian-ligusticum extract （VLE）, valeriana officinalis extract （VOE） is γ aminobutyric acid （GABA） receptor kinetin, which can relax cerebral vascular spasm; ligusticum wallichii Fr. Extraxt （LWE） can pass （hrough blood-brain barrier, enhance microcirculation of tissue and inhibit blood platelet aggregation and 5-Hydroxytryptamine （5-HT） release. OBJECTIVE： To prohe into the effects of VLE prepared with effective components on prevention and treatment of cerebral ischemie injury. DESIGN： Complete randomized, negative and positive control experiment. SETTING： Institute of Senile Medicine and Pharmacology of Tongji Med- ical College of Huazhong University of Science arid Technology. MATERIALS： The experiment was performed in Institute of Senile Medicine Pharmcology of Tonal Medical College of Huazhong University of Science and Teehnology from March 2003 to May 2004. Animals： ① Nutrient blood perfusion in brain tissue： Fifty Kunming mice were employed, which was randomized into normal group, solvent control （model） group, ligustrazine 50 mg/kg group, VLE 170 mg/kg group and VLE 85 mg/kg group, 10 mice in each one. ② Arterial-venous bypass method for thrombosis： Fifty Wistar rats were employed, which was randomized into solvent control （model） group, compound danshen （Radix Salviae Miltiorrhizae） 5 g/kg group, VLE 156 mg/kg group, VLE 94 mg/kg group and VLE 31.3 mg/kg group, 10 rats in each one. ③ Middle cerebral artery occlusion （MCAO） experiment： Sixty Wistar rats were employed, which was randomized into sham-operation group, solvent control （model） group, ligustrazine 10 mg/kg group, VLE 156 mg/kg group, VLE 95 mg/kg group and VLE 31.3 mg/kg, 10 mice in each one.④ Acute extensive cerebral ischemie experiment： Fifty Kunming mice were employed, which was randomized into normal group, solvent contml （model） group, ligustrazine 10 mg/kg group, VLE 200 mg/kg group and VLE 40 mg/kg, 10 mice in each one. METHODS： ① In the expe

关 键 词：脑缺血 微循环 缬草 川芎 

分 类 号：R742[医药卫生—神经病学与精神病学]