用组织芯片技术研究环氧合酶-2在不同亚型胃粘膜肠化生及胃癌中的表达  

The Research on Expression of the COX-2 in Various Subtypes of Gastric Mucous Membrane Intestinal Metaplasia and Gastric Cancer Using Tissue Microarray

作　　者：刘贵生[1] 龚均[1] 程鹏[1] 戴菲[1] 

机构地区：[1]西安交通大学第二医院消化科,西安市710004

出　　处：《中国肿瘤临床》2005年第19期1085-1088,1099,共5页Chinese Journal of Clinical Oncology

基　　金：卫生部临床学科重点项目资助(编号:20012130)

摘　　要：目的:研究环氧合酶-2(cyclooxygenase-2,COX-2)在不同亚型胃粘膜肠化生(intestinalmetaplasia,IM)及胃癌中的表达,探讨其预测IM恶变趋势的可能性,同时探讨COX-2表达与胃癌发生间的关系。方法:选择40例慢性萎缩性胃炎(chronicatrophicgastritis,CAG)伴IM、40例胃癌及相应癌旁组织,构建组织芯片。分别用高铁二铵/爱先蓝(HID/AB)及HE染色对IM及胃癌进行分型,然后用免疫组化检测不同亚型IM及胃癌中COX-2蛋白的表达。结果:COX-2表达阳性率在CAG伴IM灶、癌旁IM灶、肠型胃癌中分别为60.87%、75.00%和86.36%,三者间无显著性差异,但表达强度在CAG伴IM灶→癌旁IM灶→肠型胃癌顺序中呈逐渐升高趋势(P<0.005)。肠型胃癌中COX-2表达阳性率及强度均显著高于弥漫型胃癌(P<0.005)。Ⅲ型IM中COX-2表达阳性率显著高于Ⅰ、Ⅱ型IM(P<0.05),从Ⅰ型、Ⅱ型到Ⅲ型,COX-2表达强度也呈逐渐升高趋势(P<0.005)。结论:随着IM愈倾向于恶性,COX-2表达水平也逐渐增高,其有可能成为预测IM恶变趋势的有用指标。COX-2表达主要与肠型胃癌的发生有关,但在弥漫型胃癌的发生中可能也有一定作用。Objectives： To study the expression of cyclooxygenase-2 （COX-2） protein in different subtypes of intestinal metaplasia （IM） of the gastric mucous membrane and gastric carcinoma, explore the possibility of forecasting the risk of malignant potential of IM with COX-2, and to investigate the relationship between COX-2 expression and gastric carcinogenesis. Methods： A total of 40 cases of chronic atrophic gastritis （CAG） with IM, 40 cases of gastric carcinoma, and corresponding paracancerous tissues were selected, respectively, in order to construct a tissue microarray. High iron diamine/alcian blue （HID/AB） staining and Hematoxylin and Eosin （HE） staining was used to classify IM and gas- tric carcinoma, and the expression of COX-2 protein was assessed using immunohistochemistry. Results： The positive rate of COX-2 expression was 60.87%, 75.00%, and 86.36% in IM loci in CAG, IM loci in paracancerous tissues, and the intestinal-type gastric carcinoma, respectively, and there was no significant difference among them. However, the expressional intensity of COX-2 protein showed a gradually increased tendency in the sequence of IM loci in CAG→IM loci in paracancerous tissues→intestinal-type gastric carcinoma （P〈0.005）. The positive rate and intensity of the expression of COX-2protein in the intestinal-type gastric carcinoma were all significant higher compared to the diffuse-type gastric cancer （P〈0.005）. The expressional positive prevalence of COX-2 protein in type m IM was significant higher than that in type I and type Ⅱ （P〈0.05）, and the expressional intensity also showed a gradually increased tendency from type I to type Ⅲ （P〈0.005）. Conclusions： The expressional level of COX-2 increases gradually with the increase of the risk of malignancy of IM, and its expressional level may be a useful index to forecast the risk of malignant potential of IM. COX-2 expression is associated with the intestinal-type gastric carcinoma, but it might also have some role in the

关 键 词：环氧合酶-2 肠化生 胃癌 组织芯片 

分 类 号：R735.2[医药卫生—肿瘤]