宫颈鳞状细胞癌及宫颈上皮内瘤样变组织中HPV16感染与P21^(waf)、Ki67表达的关系及意义  

RELATIONSHIP BETWEEN HPV16 INFECTION AND THE EXPRESSION OF P21^(waf) AND KI67 IN CERVICAL INTRAEPITHELIAL NEOPLASIA AND SQUAMOUS CELL CARCINOMAS OF THE CERVIX UTERI.

作　　者：孙瑞芳[1] 赵富玺[1] 王晋芬[1] 陈向伟[1] 

机构地区：[1]山西医科大学大同学院,大同037008

出　　处：《现代预防医学》2005年第10期1284-1286,共3页Modern Preventive Medicine

摘　　要：目的:探讨HPV感染与P21waf、Ki67表达在人类宫颈鳞癌及其癌前病变中的关系。方法:利用组织芯片技术结合原位杂交技术和免疫组化方法研究了130例正常宫颈组织及宫颈上皮内瘤样变组织和宫颈鳞癌组织中HPV感染以及Ki67、P21waf表达的情况。结果:1)与正常宫颈组织相比,CINⅡ级、CINⅢ级、原位癌、浸润癌HPV16杂交信号阳性率显著增高(P均<0.05),且浸润癌HPV16阳性率也显著高于CIN(χ2=5.670,P=0.017);2)P21waf在浸润癌组织中的阳性表达低于正常宫颈组织(P<0.05),但与CIN相比差异无统计学意义(P=0.055)。P21waf表达与HPV16感染之间呈负相关(P<0.05,r=-0.337);3)与正常宫颈组织相比,从CINⅡ到SCC Ki67的表达都有显著差异(P均<0.05),且浸润癌与CIN相比,差异也具显著统计学意义(χ2=40.452,P=0.000)。Ki67的表达与HPV16感染无相关性(P>0.05);4)P21waf与Ki67的阳性表达在CIN演变到宫颈鳞癌的过程中呈负相关(P<0.05,r=-0.406)。结论:宫颈鳞癌的形成与HPV16的感染、P21waf的弱表达或阴性表达以及Ki67高表达有很大关系。HPV16与P21waf表达为负相关,P21waf表达与Ki67表达也为负相关。可能进一步揭示了HPV16引起宫颈鳞癌的作用机制,并且配合细胞学检查对宫颈鳞癌的早期诊断具一定参考价值。组织芯片技术是高效的研究基因及其表达产物的技术平台。Objective： To investigate the relationship between HPV 16 infection and the expression of P21^waf and Ki67 in cervical intraepithelial neoplasia （CIN） and squamous cell carcinomas （SCC） of the cervix uteri. Method： Tissue mieroarray combined with in situhybridizafion （ISH） and immunohistochemieal staining （EliVison）^tx plus method was used to detect the expression of HPV16 RNA, Ki67 and P21^waf protein in all 130 eases of normal eervieal tissue and CIN and SCC of the cervix uteri. Results： 1） The positive rate of HPV16 hibrid signals in CIN Ⅱ, CIN Ⅲ, In situ eareinoma and SCC were all signifieandy higher than in normal cervieal tissue （all P〈0.05）, and was also higher in SCC than in CIN （χ^2=5.670, P=0.017）. 2） The positive expression rate of P21^waf protein in SCC were signifieandy lower than normal cervieal tissue （P〈0.05）, but no significant difference between in SCC and in CIN （P〉0.05） . It was also found a negtive correlation between expression of P21^waf and infeetion of HPV16 （P〈0.05, r=-0.337） .3） There was a signifieant differenee in the expression of Ki67 between normal cervical tissue and CIN Ⅱ, CIN Ⅲ, In situ cancer, SCC respectively （all P〈0.05） .4） The positive expression of P21^waf and Ki67 showed negative correlation in the progress form CIN to SCC （P〈0.05, r=-0.446）. Conclusion： There are very important relationship between HPV16 infection and the negtive or poor expression of P21^waf and the high expression of Ki67 in the progress from CIN to SCC. HPV16, Ki67 show negative correlation to P21^waf. Maybe it can reveal the detailed mechanism which HPV 16 participate in malignant transformation and give some reference to improve the early diagnosis of cervical cancer. Tissue microarray is an efficient technique to study genes and their expression product.

关 键 词：宫颈鳞状细胞癌 宫颈上皮内瘤样变 人类乳头瘤病毒 P21^waf Ki67 组织芯片 

分 类 号：R730.231.3[医药卫生—肿瘤]