三氧化二砷对喉癌Hep-2细胞增殖的影响  

Effect of arsenic trioxide on proliferation of Hep-2 cell .

作　　者：陈勇[1] 蒋刈[1] 黄少鹏[1] 李瑞玉[1] 

机构地区：[1]福建省立医院耳鼻咽喉科,福建福州350001

出　　处：《现代肿瘤医学》2005年第5期616-617,共2页Journal of Modern Oncology

摘　　要：目的观察三氧化二砷(As2O3)对喉癌Hep-2细胞株增殖的影响及细胞周期的改变。方法体外培养的Hep-2细胞与不同浓度的As2O3作用24、48、72小时,通过MTT还原法检测细胞的生长抑制率,流式细胞仪检测细胞凋亡率并进行细胞周期分析。结果As2O3可有效抑制Hep-2细胞的生长,呈时间和浓度依赖性特点。2μmol/LAs2O3作用24h时,S期细胞比例增高,72小时后,S期细胞明显下降,细胞大量凋亡。As2O3在低浓度时,阻滞细胞通过S期,高浓度时诱导S期细胞凋亡。结论As2O3可抑制Hep-2细胞的增殖,与细胞周期阻滞有关。Objective To observe the proliferation and the cell cycle in laryngeal carcinoma Hep - 2 cell exposed to arsenic trioxide （ As2O3 ）. Methods The cell viability was measured by MTr reduction assay. The apoptic cells were observed and distinguished by fluorescence microscopy. Results As2O3 affected Hep-2 cell growth apparent- ly. The cell viability increased with dose and time dependence. The most prominent effect of As2O3 on cell cycle kinetics was a slowdown in S-phase transit during which cells undergo apoptosis. S -phase cell percentage increased after exposed to 2μmol/L As2O3 for 24h, with time elapsing, S-phase cell reduced. Lower concentration of As2O3 caused S - phase block, while higher concentration induced apoptosis of S - phase cell . Conclusion As2O3 can suppress growth and proliferation of Hep -2 cells , which could be related to the cell cycle arrest.

关 键 词：三氧化二砷 HEP-2细胞 细胞周期 

分 类 号：R73-351[医药卫生—肿瘤]