HPLC法测定卵磷脂中6个成分的含量  

Determination of the concentrations of 6 components in lecithin by HPLC method

作　　者：陈妙芬[1] 万丹晶[2] 华瀛夏[1] 陈刚[1] 

机构地区：[1]上海市药品检验所,上海200233 [2]复旦大学药学院放射药学教研组,上海200032

出　　处：《药学服务与研究》2005年第3期234-236,共3页Pharmaceutical Care and Research

摘　　要：目的:采用HPLC法测定卵磷脂中6个成分的含量.方法:采用LiChrosphere 100 DIOL色谱柱(250 mm×4mm,5μm),以正庚烷:异丙醇(65:114)为流动相A,正庚烷:异丙醇:水(31:62:12)为流动相B,梯度洗脱,流速0.8mL/min,用蒸发光散射检测器进行检测.结果:磷脂酰胆碱和磷脂酰乙醇胺的线性范围分别为1.69～33.76μg(r=0.997 9),0.33～6.57μg(r=0.999 6),棕榈酸甘油酯、胆固醇、鞘磷脂和溶血性磷脂酰胆碱的最低检测限分别为0.3、3、10和20 ng.结论:本方法操作简便、准确,可用于卵磷脂中各个成分的测定.Objective： To determine the concentrations of 6 components in lecithin by HPLC method. Methods： A DIOL column （250 mm×4 mm, 5μ m） was used. The gradient elution mobile phase consisted of two eluents： solvent A was n-heptane ： iso-propanol （65 ： 114） and solvent B was n-heptane ： iso-propanol ： water （31 ： 62 ： 12）. The flow rate was 0.8 mL/min and the detector was evaporative light-scattering detector（ELSD）. Results： The linear range was 1.69-33.76μg for phosphatidyl choline （r=0.9979） and 0.33-6.57 μg for phosphatidyl ethanolamine （r=0.9996）. The detection limit was 0.3 ng for tripalmitin, 3 ng for cholesterol, 10 ng for sphingomyelin and 20 ng for lysophosphatidyl choline, respectively. Conclusion： The method is rapid, accurate and reliable, and can be used to determine the components of lecithin.

关 键 词：卵磷脂 磷脂酰胆碱 磷脂酰乙醇胺 色谱法 高压液相 蒸发光散射检测器 

分 类 号：R977.6[医药卫生—药品]