TNF与卡铂联合应用对A_(549)肺癌细胞系细胞毒作用的研究  

Cytotoxic Study of TNF Combined with CBDCA on A_(549)Lung Cancer Cell

作　　者：刘晓翌[1] 舒清波[1] 张华亭 江曼[1] 杨飞[1] 王敏[1] 余峰 

机构地区：[1]湖北医科大学基础医学院病理学教研室,武汉430071

出　　处：《湖北医科大学学报》1996年第2期120-122,共3页

摘　　要：采用＾３Ｈ－ＴｄＲ掺入试验及台盼蓝活细胞拒染法观察了肿瘤坏死因子（ＴＮＦ）与卡铂联合应用对Ａ５４９肺癌细胞的细胞毒作用，实验分为空白对照组，ＴＮＦ组（５００Ｕ／ｍｌ），卡铂组（４０μｇ／ｍｌ）ＴＮＦ（５００Ｕ／ｍｌ）加卡铂（４０μｇ／ｍｌ）组＾３Ｈ－ＴｄＲ掺入试验结果表明，联合用药增强了药物对Ａ５４９细胞ＤＮＡ合成的 制作用，抑制率为６９．３％，而ＴＮＦ组与卡铂组的抑制率则分别为２５．１％和５６．Using ~3H-TdR incorporation assay and the method of living cell count,we have observed the cytotoxicity of TNF combined with CBDCA on A_(549)lung cancer cells.The A_(549)lung cancer cells were randomly divided into four groups:Group A was cultured in the drug-free medium for control.Group B,C and D were cultured in the medium including TNF(50μg/ml),CBCDA (40μg/ml),TNF(500U/ml)and CBDCA(40μg/ml)respectively.The result of ~3H-TdR incor- poration assay indicated that Group D increased the rate of DNA inhibition(69.3%),the rate of DNA inhibition of Group B and Group C were 25.1% and 56.4% respectively.The result of living cell count was that the living ceils of Group C and Group D were marked fewer than Group A and Group B,and the cytotocixity of the former had a time-dependent.Though the living ceils of Group B decreased,the anticancer effect was not very strong and the cells count were increased slowly after the first 24 hours.The research indicated that the anticancer mech- anism of TNF and CBDCA was different.CBDCA is the cytotoxic effect.TNF is the effect of growth inhibition.Using the drug combination increased the sensitivity of A_(549)cells to TNF and CBDCA.

关 键 词：肺肿瘤 细胞毒性 A549细胞 卡铂 肿瘤坏死因子 

分 类 号：R734.205[医药卫生—肿瘤]