地塞米松对人乳腺癌细胞系MCF-7细胞增殖及细胞周期的影响  

Effects of dexamethasone on proliferation and cell cycle of human breast cancer cell line MCF-7

作　　者：徐明娟[1] 惠宁[1] 刘宇健[2] 

机构地区：[1]第二军医大学长海医院妇产科,上海200433 [2]第二军医大学基础医学部病理生理学教研室,上海200433

出　　处：《第二军医大学学报》2005年第9期1015-1017,共3页Academic Journal of Second Military Medical University

摘　　要：目的:观察不同浓度的地塞米松(Dex) 对人乳腺癌细胞系(MCF-7) 细胞增殖、细胞周期分布及P21/WAF1表达的影响.方法:以不同浓度Dex(10-10～10-6 mol/L)处理细胞,采用活细胞计数法观察细胞增殖情况;测定碱性磷酸酶(AKP)活性;通过流式细胞仪技术,测定Dex作用后其细胞周期分布;通过蛋白印迹分析的方法检测Dex对p21/WAF1表达的影响.结果:Dex对MCF-7细胞的增殖有明显抑制作用, 10-7mol/L Dex作用5 d后活细胞计数为对照组的70%,细胞在G0/G1期停滞; Dex还可使MCF-7 细胞AKP活性增高,这种作用具有时间和剂量依赖性;Dex对p21/WAF1表达无明显影响.结论:Dex对MCF-7细胞有明显的增殖抑制作用,细胞在G0/G1期停滞.Objective：To study the effects of dexamethasone （Dex）, a synthetic glueoeortieoid, on the proliferation and cell cycle of human breast carcinoma cell line MCF-7. Methods： MCF-7 cells were treated with different concentrations of Dex （10^-10 10^-6 mol/L）. Cell proliferation was evaluated by viable cell count and alkaline phosphatase（AKP） activity was also determined. The cell cycle was examined with flow cytometry and p21/WAF1 expression was detected with Western blot analysis. Results： Dex inhibited the proliferation of MCF 7 cells, with the cell number being 70% of the control group 5 d after treatment with Dex（10^-7 mol/L）. Dex also induced G0/G1 arrest of MCF-7 cells phase. AKP activity increased significantly af ter treatment with Dex in a time and dose-dependent manner. Dex had no obvious effect on p21/WAF1 expression. Conclusion： Glucocortocoids can obviously inhibit the proliferation of MCF-7 cells and induce G0/G1 phase arrest.

关 键 词：地塞米松 乳腺肿瘤 细胞增殖 细胞周期 P21/WAF1 

分 类 号：R737.9[医药卫生—肿瘤]