产紫杉醇菌株原生质体诱变育种的研究  

Study on Breeding up High-yield Strain of Taxol by Protoplast Mutagensis

作　　者：赵凯[1] 周东坡[1] 平文祥[1] 刘军[1] 马玺[1] 金涛[1] 

机构地区：[1]黑龙江大学生命科学学院

出　　处：《生物工程学报》2005年第5期848-851,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.);黑龙江省"十五"重大攻关项目(No.GA02C101);哈尔滨市重点项目(No.011421126)资助。~~

摘　　要：对紫杉醇产生菌NCEU_1的原生质体进行了紫外线和氯化锂复合诱变,筛选制霉菌素抗性突变株,共筛选出了4株正突变株。经发酵筛选试验,获得了一株遗传性状稳定、高产紫杉醇的原生质体诱变菌株———UL04-5,其紫杉醇产量从出发菌株的314.07μgL提高至418.24μgL。In order to obtain resistant mutants to nystatin, ultraviolet radiation and LiCl were used to mutagenize the protoplasts of taxol-producing fungi NCEU-1, and four positive mutants with high yield of taxol were screened out on nystatin flat. After further screening experiments on ferroentation, a mutant strain--UI04-5 which was able to produce taxol with high yield and could be stably passed on in genetics was eventually found, it＇s ability to produce taxol was improved from 314.07μg/L（strain NCEU-1）to 418.24μg/L（ strain UI04-5 ） .

关 键 词：紫杉醇 紫杉醇产生菌 原生质体诱变 

分 类 号：TQ464[化学工程—制药化工]