洋葱伯克霍尔德菌CF-66抗菌物质的分离纯化及性质的研究  

Purification and properties of anitibiotic from Burkholderia cepacia CF-66

作　　者：权春善[1] 郑维[1] 曹治明[1] 王军华[2] 范圣第[1] 

机构地区：[1]大连民族学院生命科学学院,大连116600 [2]大连理工大学环境与生命学院,大连116024

出　　处：《微生物学报》2005年第5期707-710,共4页Acta Microbiologica Sinica

基　　金：辽宁省自然科学基金项目(2040949)~~

摘　　要：洋葱伯克霍尔德菌CF-66能够抑制立枯丝核菌等若干植物病原菌和其它一些真菌的生长。CF-66菌发酵液的粗提液通过Sephadex-75pg、Sephacryl S-100柱层析分离纯化,获得抗菌物质CF66I。此抗菌物质耐热性强,耐碱,但在强酸性条件下不稳定。低浓度有机溶剂的存在有利于抑菌活性的提高。对其结构的研究表明CF66I是以(CH2CH2O)n为主要单元结构并带有酰氨键的化合物。Burkholderia cepacia CF-66 isolated from compost showed antifungal activity against plant pathogens such as Rhizoctomia solani and some other fungi. CF66I produced by Burkholderia cepacia CF-66 was separated by gel chromatography using Sephadex-75pg and Sephacryl S-100 column. CF66I has very high degree of stability of antifungal activity under higher temperature and alkaline conditions. Some organic solvents could improve its activity at low concentration. The research for the structure of CF66I find that it is a compound with amide bonds and its main structural units is （CH2CH2O）n.

关 键 词：洋葱伯克霍尔德菌 分离纯化 抑菌活性 

分 类 号：S482.292[农业科学—农药学]