甜樱桃矮化砧木试管苗离体生根条件的优化  

The Optimization Rooting Condition of Cherry Dwarf Rootstock In vitro

作　　者：黄文江[1] 周守标[1] 马锋旺[2] 邵好好[3] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000 [2]西北农林科技大学园艺学院,陕西杨凌712100 [3]宁波大学生命科学与生物工程学院,浙江宁波315211

出　　处：《南京林业大学学报（自然科学版）》2005年第5期53-56,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：安徽省教育厅自然科学资金资助项目(2003KJ162);安徽师范大学青年资金资助课题(2003xqn09)

摘　　要：以甜樱桃矮化砧木Gisela系品种为试材,对影响试管苗生根的因素进行了研究。结果表明,培养基中无机盐浓度、外源激素水平、种类以及外植体的发育状况等对试管嫩茎生根具有明显影响。取继代次数超过30次、生长旺盛、叶小呈嫩黄绿色的嫩茎,接种在1/4MS添加0.5 mg/L IBA的诱导生根培养基上,黑暗处理15 d,然后移至光下诱导生根,结果发现生根率高,且根生长快、根量多、移栽易于成活,矮化砧木Gisela5,Gisela6离体生根率可达83.3%和86.9%。Factors influencing rooting of tissue cultured shoots from cherry dwarf rootstock, Gisela5 and Gisela6 （Prunus cereasus×P, canescens） were studied. The result showed that the successful rooting system was closely related to the concentration of macronutrient of MS medium, auxin concentration and developing state of the explant. Jender shoots with smaller leaves and subcultured more than 30 times were cultured on the medium with 1/4 MS macronutrient and IBA 0. 5 mg/L for 15 d under dark condition. The highest rooting rate was gained from shoots of Gisela5 and Gisela6, which counted for 83.3% ,86.9% respectively.

关 键 词：甜樱桃矮化砧木 离体 生根 

分 类 号：S723[农业科学—林木遗传育种]