五倍子降血糖有效组分的药理筛选  

Pharmacological screening of hypoglycemic fractions from Chinese gall

作　　者：蒲旭峰[1] 杨奎 侯世祥[1] 

机构地区：[1]四川大学华西药学院 [2]成都中医药大学,四川成都610072

出　　处：《中国药学杂志》2005年第19期1470-1473,共4页Chinese Pharmaceutical Journal

摘　　要：目的筛选中药五倍子降血糖作用的有效组分,比较提取工艺对降血糖活性的影响。方法采用不同极性溶剂提取并结合化学处理与柱色谱分离,获得五倍子各不同组分,以四氧嘧啶致家兔高血糖模型及链脲佐菌素致小鼠糖尿病模型对各组分的降血糖活性进行比较;并以对链脲佐菌素所致小鼠糖尿病模型的影响为依据,比较提取工艺对降血糖活性的影响。结果五倍子石油醚提取物有明显的降血糖活性,其中脂肪油类成分为降血糖有效组分;进一步的化学与柱色谱分离均导致降血糖活性降低;溶剂提取与CO_2超临界提取所得五倍子油降血糖活性无明显差异(P>0.05)。结论五倍子石油醚提取物有明显降血糖作用,其有效组分为其脂肪油。OBJECTIVE To screen the effective fractions from Chinese gall for the treatment of diabetes mellitus, and to compare the extraction processes.METHODS After gaining different fractions from Chinese gall by extracting with the solvents of different polar, and with the help of chemical methods and column chromatographic techniques, studies were conducted to compare the hypoglycemic activities of these fractions in hyperglycemic rabbits and mice respectively induced by tetraoxypyrimidine and steptozotocin. On the basis of the latter animal the model, the influences of the extraction processes on the hypoglycemic activities were compared. RESULTS The petroleum ether extract showed obvious hypoglycemic activity. The fatty oils were the effective constituents. Further isolation with chemical and column chromatographic methods decreased the hypeglycemic activity. The fatty oils of Chinese gall gained by solvent extraction and supercritical CO2 fluid extraction, showed no significant differences in hypoglycemic activities（ P 〉 0.05）. CONCLUSION The petroleum ether extract has obvious hypoglycemic activity.The fatty oils are the effective constituents.

关 键 词：五倍子 五倍子油 药理筛选 降血糖 糖尿病模型 

分 类 号：R285.5[医药卫生—中药学]