基于株平均值的胚乳性状QTL作图的极大似然方法  

Plant Average-based Maximum Likelihood Mapping of Quantitative Traits Loci Controlling Endosperm Traits

作　　者：徐辰武[1] 王伟[1] 胡治球[1] 孙长森[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室,江苏扬州225009

出　　处：《作物学报》2005年第10期1271-1276,共6页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(39900080;30270724和30370758)联合资助

摘　　要：根据三倍体胚乳性状的数量遗传模型,发展出一种新的专用于胚乳性状数量基因座位(QTL)区间作图的统计方法。该方法以分离群体中各植株的分子标记基因型以及植株上若干粒种子胚乳性状的平均值为数据模式,采用基于平均值混合分布理论的极大似然方法进行QTL分析。QTL效应估计通过EM算法实现。由于该方法利用标记基因型内QTL基因型的混合分布特性,因此,它比同样基于株平均值的最小平方QTL分析方法以及迭代重新加权最小平方QTL分析方法具有更高的统计功效和精确度。方法的可行性和有效性通过计算机模拟数据分析得到了进一步验证。Endosperm traits are important in determining grain quality in cereals. Endosperm, which is derived from two polar nuclei fusing with one sperm, is a triploid tissue whose genetic constitution is more complex than common diploid tissue. Current statistical methods for mapping quantitative trait loci （QTL） under diploid genetic control have not been effective for dealing with endosperm traits because of the complexity of their triploid inheritance. Based on the quantitative genetic models for triploid endosperm traits and mixture distribution theory, a new maximum likelihood method implemented via expectation-maximization algorithm which included the exact and approximate EM algorithms for mapping of quantitative trait loci （QTL） underlying endosperm traits was proposed in this paper. The method used the DNA molecular marker genotypes of each plant in segregation population such as F2, backcross etc., and the plant average of several endosperms of each plant as data set to map QTL. Because the method considered the mixture distribution property of QTL genotypes within flanking marker genotypes, it was expected that the proposed method not only increased the statistical power of QTL mapping but also improved the accuracy and precision of QTL effect estimates. Efficiency and feasibility of the methods have been verified through Monte Carlo simulation studies. The means and standard deviations of the estimated QTL effects and locations as well as the empirical powers under different QTL heritabilities and sampling strategies were listed in Table 1. Results of simulation showed that： （ 1 ） Whether the exact EM algorithm or the approximate EM algorithm, the method may provide accurate estimates of beth the QTL effects and locations with very high statistical power; （2） Though the exact EM algorithm can＇ t significantly increase the statistical power of QTL detecting and the accuracy of QTL locations compared with the approximate EM algorithm, it may apparently increase the precision of QTL locations

关 键 词：胚乳性状 三倍体 区间作图 极大似然估计 EM算法 

分 类 号：Q943[生物学—植物学]