毛叶茶提取物对不同细胞生长的影响及体内协同抗瘤作用  

THE EFFECT OF ECPC ON THE GROWTH OF VARIOUS CELLS AND IN VIVO SYNERGISM OF ITS ANTITUMOR EFFECT

作　　者：刘宗潮[1] 谢冰芬[1] 潘启超[1] 苏秀容[1] 冯公侃[1] 王理开[1] 张润梅[1] 张宏达[1] 

机构地区：[1]中山医科大学肿瘤研究所,中山医科大学生物化学教研室,中山大学生物系植物研究室

出　　处：《癌症》1996年第3期164-167,共4页Chinese Journal of Cancer

基　　金：中央卫生部科学基金

摘　　要：应用噻唑兰（ＭＴＴ）法测得毛叶茶提取物（ＥＣＰＣ）对人肝癌细胞（ＢＥＬ－７４０２）的半数抑制浓度（ＩＣ＿（５０））为３５１．１μｇ／ｍｌ；台盼蓝拒染法（ＴＢＥ）的ＩＣ＿（５０）为１１５．２μｇ／ｍｌ；用ＭＴＴ法测得ＥＣＰＣ对人红白血病细胞（Ｋ＿（５６２））的ＩＣ＿（５０）＞１０００μｇ／ｍｌ，ＴＢＥ法测得其对Ｋ＿（５６２）细胞的ＩＣ＿（５０）为８１．４μｇ／ｍｌ。ＥＣＰＣ对人胎儿肺纤维细胞（ＨＦＬＦ）和人胎儿肾细胞（ＨＦＫ）显示促生长作用。在药物浓度为６２．５、１２５．０、２５０．０、５００．０和１０００．０μｇ／ｍｌ的培养下，对ＨＦＬＦ细胞的生长促进率分别为—１０．４７、＋１５．４８、＋３１．９１、＋５３．０２和＋８３．３７％：对ＨＦＫ细胞的生长促进率分别为＋１４．１８、＋２６．６１、＋５２．６２、＋８８．１３和＋１０３．７１％。ＦＣＰＣ分别与阿糖胞苷（Ａｒａ—Ｃ）、环磷酰胺（ＣＴＸ）和阿霉素（ＡＤＭ）合用对网织细胞肉瘤（Ｌ＿２）的抑瘤率高于各个单药的抑瘤率。加用ＥＣＰＣ后Ａｒａ—Ｃ可从单用平均抑瘤率３８．２％升高到６４％；ＣＴＸ从平均３１．３％升高到４８．９％；ＡＤＭ从平均１５．２％升高到４５．５％，ｑ?The cytotoxic effect of extract of Camellia ptilophylla Chang(ECPC)on human hepatic can-cer cell line(BEL-7402)and human erythroleukemia cell line(K562)was determined using MTT assaymethod and tyrpan blue exclusion method(TBE).The IC50 of ECPC against BEL-7402 cells was calculat-ed to be 351.1μg/ml using MTT assay and 115.2 μg/ml using TBE assay. The IC50 against K562 cells wasover 1,000 μg/ml using MTT assay and 81.4μg/ml using TBE assay. While ECPC on human fetus lung fi-brocyte(HFLF)and human fetus kidney cells(HFK)showed growth promotion effect.Under the concen-tration of ECPC of 62.5,250.500 and 1,000g/ml,the growth promotion rates on HFLF were -10. 47,+15.48,+ 31.91,+ 53.02 and + 83.37%,respectively,The growth promotion rates of ECPC on HFKwere + 14.18,+ 26.61,+ 52.62,+ 88. 13 and + 103.71 %. The inhibitory rates of the combination of ECPC with Ara -C or CTX or ADM against L2 in mice werehigher than those of respective single drug. The average inhibitory rates(IR)of Ara -C after addition of ECPC was increased from 38.2% to 64%. The IR of CTX after addition of ECPC was increased from31.3% to 48.9%,and the IR for ADM from 15.2% to 45.5%,q all > 1,showing that the combination ofECPC with these drugs could enhance the antitumor effect.In human nasopharyngeal cancer cells(CNE2)treated by ECPC of concentration of 125-500 μg/ ml DNA breakage was induced.The degree of DNAbreakage increased with increasing concentration of ECPC.

关 键 词：毛叶茶提取物 增效作用 DNA 抗肿瘤作用 

分 类 号：R73－36[医药卫生—肿瘤]