α－双炔失碳酯诱导分化作用的形态定量研究  

MORPHOMETRIC STUDY ON THE DIFFERENTIATION-INDUCING EFFECT OF a-ANORDRIN

作　　者：林屹[1] 周佩琴[1] 胥彬 

机构地区：[1]中国科学院上海药物所

出　　处：《癌症》1996年第3期178-180,共3页Chinese Journal of Cancer

摘　　要：应用自动化图像分析仪对低剂量α－双炔失碳酯（ＡＮＯ）诱导ＨＬ－６０细胞分化的作用进行了形态测量研究。４μｇ／ｍｌＡＮＯ连续作用ＨＬ－６０细胞５天。共测８个形态指标。结果表明，细胞面积：核面积，核周长，核最大直径，核最小直径，核灰度／核面积随药物作用时间增加而增大，而核浆比却随时间增加而减小。所有数据表明，在低剂量ＡＮＯ（４μｇ／ｍｌ）作用下，ＨＬ－６０细胞在１～４天内作为细胞生命基本的ＤＮＡ变化显著，但细胞形态基本稳定。第５天开始呈现显著性地向成熟细胞转化的倾向。The effect of a-Anordrin(ANO)on the induction of differentiation of the human promyelo-cytic leukemia cells line(HL-60)was studied morphometrically by automatic image analyser,Cells werecontinuously exposed to low doses(4μg/ml)of ANO for 5 days,Eight morphometric pararneters related tothe quantitiative analysis were examined.The results showed that after administration of ANO(4μg/ml)the cellular area,nuclear area, nuclear perimeter,maximal nuclear diameter,minimal nuclear diameter,form factor and grey-nuclear area ratio weve markedly increased,whilst nucleus-cytoplasm ratio decreased.These data indicated that DNA content of HL-60 cells was altered significantly during the first 4 days afteradminstration of a-anordin. On the fifth day,the morphological change could be seen and HL-60 cellswere differentiated obviously into mature cells.

关 键 词：Α-双炔失碳酯 肿瘤 细胞分化 形态定量分析 

分 类 号：R73－3[医药卫生—肿瘤]