鼻咽癌体外药物敏感性试验方法的初步研究  

PRIMARY STUDY OF IN VITRO DRUG SENSITIVITY METHOD FOR NASOPHARYN GEAL CARCINOMA

作　　者：梁永钜[1] 潘启超[1] 麦伟源[1] 闵华庆[1] 

机构地区：[1]中山医科大学肿瘤研究所,中山医科大学肿瘤医院

出　　处：《癌症》1996年第3期216-218,共3页Chinese Journal of Cancer

摘　　要：作者建立了一个可以纤维镜采集的小量鼻咽癌组织进行试验的体外药敏试验方法。本法采用滤纸支持的组织块培养法和ＭＴＴ终点，以图像分析法测定瘤块中甲蓝染面积来确定药物对肿瘤细胞的作用。结果显示抑制率随药物浓度增加而上升，结果可重复。不同个体的癌组织对药物的反应差异较大，在ＤＤＰ浓度为２．５、５及１０μｇ／ｍｌ时，抑制率≥５０％的例数分别为０／６、６／１１、９／９；在５ＦＵ浓度为２０、１００及５００μｇ／ｍｌ时，抑制率≥５０％的例数分别为１／１０、４／１０、７／１０；在平阳霉素浓度为１０、５０及２５０μｇ／ｍｌ时，抑制率≥５０％的例数分别为３／１１、８／１１、９／１１。可采用ＤＤＰμｇ／ｍｌ、５ＦＵ１００μｇ／ｍｌ和平阳霉素１０μｇ／ｍｌ为监界浓度以鉴别肿瘤的敏感性。本法保留了实体瘤组织结构各种影响药物作用的因素，简捷易行，可望成为实用的微量化体外药敏试验方法用于临床。We developed an in vitro drug sensitivity test method that can use small size tissues of na- sopharyngeal carcinoma obtained by fibroendoscope. It applies the tissue fragment culture supported by filterpaper and uss MTT method and formazan as end point.The effect of drugs on tumor cell was determined bymeasuring the formazan blue area in tumor fragments with image analysis.It was shown that the inhibitionrate(IR )increased with incresasing drug concentration,the results had reproductivity and the responses ofdifferent individual cancer tissues varied widely. At DDP 2.5,5 and 10μg/ml,the ratios of IR≥50%were0/6,6/11,9/11 respectively;at 5FU 20,100 and 500 μg/ml,the ratios of IR≥50% were1/10,4/10,7/10 respectively;at pingyangmycin 10, 50 and 250μg/ml,the ratios of IR≥50% were 3/11,8/11,9/11,re- spectively. The cut off concentrations of DDP, 5FU and pingyangmycin were 5,100 and 10 μg/ml respec- tively. This method maintains the tissue architectue of solid tumor.It is simple and is expected to be clini- cally useful.

关 键 词：鼻咽癌 药物疗法 药敏试验 体外 

分 类 号：R739.63[医药卫生—肿瘤]