油菜籽中主要硫甙的提纯与抗肿瘤活性  

Purification and Antitumor Activity of Major Glucosinolates Separated from Brassica Oleracea Rapeseed

作　　者：周锦兰[1] 俞开潮[1] 

机构地区：[1]华中科技大学化学系,武汉430074

出　　处：《应用化学》2005年第10期1075-1078,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(No.20275011);湖北省自然科学基金(2002AB051)资助项目

摘　　要：甘蓝型(Brassica oleracea)油菜籽中硫甙混合物用70%甲醇提取后,依次通过酸性氧化铝色谱柱和反相碳十八硅胶柱分离提纯,得到1-硫[(1Z)-3-羟基-1-[(磺氧基)亚氨基]-4-戊烯基]-1-硫代--βD-葡萄糖钾盐(progoitrin)。表征了progoitrin的结构,测定了progoitrin的纯度。并测试了用硫甙混合物及纯化硫甙作为化学抗癌剂的抗肿瘤活性。实验结果显示,混合硫甙和纯化硫甙的降解产物对小鼠S180实体瘤均具有抑制作用,按60 mg/kg硫甙剂量给药时,抑瘤率分别达到67.3%和59.5%。A procedure for the preparative isolation of 1-S-[ （1Z）-3-hydroxy-l-[ （sulfooxy） imino]-4-pentenyl]-1-thio-β-D-glucopyanose, potassium salt（progoitrin） from Brassica oleracea rapeseed is reported.The glucosinolates were extracted from Brassica oleracea rapeseed with a 70% methanol solution; then, the extractives were separated and purified to get the glucosinolates on chromatographic column with alumina sup- port; and finally, the glucosinolates were purified by reverse-phase octadecyl （C18） silica support, and progoitrin was obtained as the major content of glucosinolate. The structure of progoitrin was identified on the basis of its physicochemical properties, UV, IR, ~H NMR and elemental analysis. Its purity was determined by high performance liquid chromatographic （HPLC） method. The anticarcinogenic effects of mixed glucosinolate and pure progoitrin were measured. The experimental results show that when a dose of 60 mg/kg was administrated, the ratio of inhibitory to S^s0 sarcoma was 67.3% and 59.5% for mixed glucosinolates and pure progoitrin, respectively. This study suggests that both the mixture of glucosinolates and pure progoitrin in Brassica oleracea rapeseed have potential anticarcinogenic effect as chempreventive agents.

关 键 词：硫代葡萄糖苷 分离提纯 S180 抗肿瘤作用 

分 类 号：O629.1[理学—有机化学]