渤海红鳍东方豚体内产毒细菌的微生态分布及B3B菌株的生物学特性  

Microecological distribution of toxin-producing bacteria in Fugu rubripes in Bohai Sea and biological characteristics of B3B

作　　者：范延辉[1] 胡江春[1] 马成新[1] 薛德林[1] 刘丽[1] 李延茂[1] 王书锦[1] 张荣庆[2] 

机构地区：[1]中国科学院沈阳应用生态研究所 [2]清华大学,北京100084

出　　处：《应用生态学报》2005年第10期1952-1955,共4页Chinese Journal of Applied Ecology

基　　金：国家"863"计划资助项目(2002AA625020).

摘　　要：对我国渤海红鳍东方豚(Fugurubripes)体内产毒细菌的微生态分布进行了考察.结果表明,渤海红鳍东方豚体内的各部位都普遍存在着产毒细菌,从其卵巢、肝脏等组织中分离到了19株可产强毒的菌株,对其中B3B菌株进行进一步研究鉴定,生理生化试验及16SrDNA序列测定结果表明,该菌属于芽孢杆菌属(Bacillus).同时,对其发酵产物进行分离精制后,通过小鼠试验、薄层层析试验及质谱分析,初步认定发酵产物中含有河豚毒素(Tetrodotoxin,TTX).The study showed that toxin-producing bacteria were commonly existed in each part of Fugu rubripes body, 19 strains of high poisonous bacteria were isolated from its ovary, liver and other tissues, among which, strain B3B was identified as genus Bacillus based on its physiological and biochemical characteristics and 16SrDNA sequence, and could produce tetrodotoxin through mouse test, thinner chromatography, and mass spectrography.

关 键 词：红鳍东方豚 微生态 发酵产物 河豚毒素 

分 类 号：Q938[生物学—微生物学]