10株新城疫病毒广西分离株HN蛋白基因的克隆与序列分析  

Cloning and sequence analysis of HN gene of ten Newcastle disease virus isolates from Guangxi

作　　者：刘加波[1] 谢芝勋[1] 唐小飞[1] 庞耀珊[1] 邓显文[1] 

机构地区：[1]广西兽医研究所,广西南宁530001

出　　处：《畜牧与兽医》2005年第7期6-10,共5页Animal Husbandry & Veterinary Medicine

基　　金：广西科技攻关项目(桂科攻0235001-4);广西留学基金项目(桂科回0236005);广西水产畜牧局科研计划资助

摘　　要：根据基因库(GenBank)新城疫病毒(NDV)的HN基因序列设计了2对特异性引物,应用RT-PCR技术对广西在2000～2003年暴发新城疫的鸡群中分离的10株NDV毒株的HN基因进行了扩增,扩增产物克隆并测序,拼接出10个NDV广西分离株的HN基因全序列,其序列全长均为1 713 bp,编码571个氨基酸,均有13个半胱氨酸残基.其中GX8/03有6个糖基化位点,而GX2/00、GX6/02、GX7/02和GX5/00有5个糖基化位点,GX1/00、GX3/00、GX4/00和GX9/03有4个糖基化位点.除GX5/00和GX10/03分离株外,其他8个NDV分离株在HN基因抗原位点Ⅰ发生变异,即347位由谷氨酸(E)被甘氨酸(G)替代,GX8/03分离株在HN基因抗原位点Ⅱ的495位由赖氨酸(K)替代谷氨酸(E).与11株已发表的NDV HN基因全序列相比较,其核苷酸同源性在79.6%～ 97.9%之间,推导的氨基酸同源性在87.2%～98.1%之间.Two pairs of special primers were designed and synthesized according to the HN protein gene sequences of Newcastle disease virus （NDV） strains from GenBank. HN genes of ten NDV isolates from chicken farms in Guangxi between 2000 to 2003 were amplified by reverse transcription-polymerase chain reaction （RT-PCR） technique. The HN genes were sequenced after the products of amplification being cloned, and the whole sequences of HN genes from ten NDV Guangxi isolates were obtained. The full length was 1 713 bp, which encoded 571 amino acids and contained 13 cycteine residues. The predicted amino acids sequences of GX8/03 contained 6 glycosylation sites; GX5/ 00, GX6/02, GX7/02 and GX10/03 contained 5 glycosylation sites; the predicted sequences of GX1/00, GX3/00, GX4/00 and Gxg/03 contained 4 glycosylation sites. Except for GX5/00 and GX10/03 NDV isolates, the antigenic site Ⅰ of HN gene from the other 8 NDV isolates was changed in 347 site amino acid from Glu to Gly. The antigenic site Ⅱ of HN gene from GX8/03 NDV isolates was changed in 495 site amino acid from Lys to Glu. Comparison of ten NDV HN protein sequences with 11 other published sequences revealed that the homology of the nucleotide was 79.6% -97.9% and the homology of the deduced amino acid sequences was 87.2% -98. 1%.

关 键 词：新城疫病毒 HN蛋白基因 克隆 序列分析 

分 类 号：S855.3[农业科学—临床兽医学]